小麦白粉菌ISSR分子标记体系构建及其分离菌株的多样性分析  

Establishment of ISSR-PCR reaction system for Blumeria graminis f.sp. tritici and its application in diversity analysis of this pathogen

作　　者：贾少锋[1,2] 段霞瑜[1] 周益林[1] 鲁国东[2] 王宗华[2] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100094 [2]福建农林大学植物保护学院,福州350002

出　　处：《植物保护学报》2007年第5期493-499,共7页Journal of Plant Protection

基　　金：国家"973"课题(2006CB100203);国家"十一五"科技支撑计划课题(2006BAD08A05);公益性行业科研专项(nyhyzx07-048)

摘　　要：为了对小麦白粉菌群体的多样性进行研究,构建了其ISSR分子标记体系。以小麦白粉菌基因组DNA为模板,用正交设计和单因素水平优化的方法对ISSR反应程序中的一些重要参数进行摸索和优化,建立了小麦白粉菌ISSR-PCR反应的优化反应体系;对20个ISSR引物的退火温度进行了优化,并筛选出一些多态性较好的ISSR引物。对33个分离菌株的ISSR扩增表明,ISSR标记在我国小麦白粉菌中存在较高的多态性;对ISSR标记揭示的白粉菌的遗传多样性和毒性多样性进行了比较,结果表明两者之间存在一定程度的相关性。In order to detect the diversity in populations of Blumeria graminis f. sp. tritici （ Bgt）, a orthogonal design and a single factor test, were used to optimize ISSR-PCR amplification system at different levels of five factors （Taq DNA polymerase, Mg^2＋ , dNTP, primer and DNA template） and a suitable ISSR-PCR reaction system was established. The optimal annealing temperature of 20 primers was confirmed by gradient PCR and polymorphic primers were screened. Polymorphic bands of 33 isolates generated by primer BDB （CA） 7 indicated that high diversity is conserved in DNA of Bgt. The dendrograms based on ISSR results and isolate virulence were also compared, showing that there is some relationship between DNA diversity and the diversity of the virulence.

关 键 词：小麦白粉菌 ISSR分子标记体系 多样性分析 毒性 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]