维生素E缺乏对雏鸡淋巴细胞膜抗氧化及膜流动性的影响  

The Effect of Vitamin E Deficiency on Antioxidation and Fluidity of Lymphocyte Membrane in Chicken

作　　者：王奔[1] 李艳飞[1] 封家旺[1] 张金龙[1] 

机构地区：[1]东北农业大学动物医学院,哈尔滨150030

出　　处：《中国畜牧兽医》2007年第11期20-22,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金项目(30471279);黑龙江省博士后科研启动金(LBH-Q05024)

摘　　要：用低维生素E(VE)和添加不饱和脂肪酸的日粮饲喂雏鸡,建立VE缺乏雏鸡模型。分离全血淋巴细胞并制备细胞膜,检测淋巴细胞膜超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量;采用荧光偏振法检测淋巴细胞膜流动性。结果表明,VE缺乏雏鸡淋巴细胞膜SOD和GSH-Px活力及膜流动性低于对照组,MDA含量高于对照组,组间差异显著或极显著(P<0.05、P<0.01)。说明VE对维持雏鸡淋巴细胞膜功能起重要作用。The activity of SOD and GSH-Px and the level of MDA of lymphocyte membrane in the modle chicken with Vitamin E （VE）deficiency that produced by using low content of VE feedstuff and supplying unsaturated fatty acid in the diet Were detected by Test Kit;the membrane fluidity of lymphocyte in the same modle chicken was detected through fluorescence polarization. The results showed that activity of SOD and GSH-Px and fluidity of lymphocyte membrane were significantly lower in experimental chicken than control ones（P〈0.05, P〈0.01）; the MDA content were significantly higher than control ones （P〈0.05）. The results indicated that VE plays an important role in maintaining the function of lymphocyte membrane in chicken.

关 键 词：维生素E 雏鸡 淋巴细胞膜 抗氧化 膜流动性 

分 类 号：S816.7[农业科学—饲料科学]