自发性高血压大鼠肾上腺髓质素2水平的变化  

Adrenomedullin2 in SHR

作　　者：袁鹰[1] 曾强[1] 王曦[1] 吴红梅[1] 齐永芬[2] 唐朝枢[2] 

机构地区：[1]中国人民解放军总医院老年心血管二科,北京100853 [2]北京大学第一医院心血管病研究所

出　　处：《中华高血压杂志》2007年第11期941-945,共5页Chinese Journal of Hypertension

基　　金：国家自然科学基金(No.30570738)

摘　　要：背景肾上腺髓质素2(ADM2)是2004年2月由日本学者所报道的,被认为是降钙素/降钙素基因相关肽超家族的一个新肽。这与2003年11月美国斯坦福大学Dr.Roh等人发现的垂体中叶素在核苷酸和氨基酸序列上完全一致,二者被认为是同物异名。该家族的已有成员包括肾上腺髓质素、降钙素基因相关肽、降钙素和胰岛淀粉样多肽。目前研究结果表明,肾上腺髓质素2参与了心血管的调节。目的研究自发性高血压大鼠(SHR)血浆ADM2[即:垂体中叶素(IMD)]的含量和在组织中水平的升降、基因表达的变化、血浆ADM2含量与血压和心质量/体质量(HM/BM)的相互关系。方法11周龄的雄性SHR8只为实验组,同龄的雄性WistarKyoto(WKY)大鼠8只为对照组。放射免疫分析方法测定血浆和组织中ADM2的含量;RT-PCR方法测定组织中ADM2的mRNA表达;心室导管方法测定血压和心功能的变化。结果1)与WKY大鼠相比,SHR的血浆、心肌和主动脉中ADM2的含量显著增高,分别为50.0%[(317.2±25.2)vs(211.4±15.0)ng/L,P<0.01]、46.5%[(293.6±34.7)vs(200.4±21.6)ng/g,P<0.05]和32.1%[(1009.0±50.1)vs(763.8±35.0)ng/g,P<0.01];2)SHR心肌和主动脉中ADM2的mRNA水平高于WKY大鼠,分别升高76.11%[(0.75±0.33)vs(0.46±0.26)%,P<0.05]和171.1%[(1.43±0.36)%vs(0.66±0.35)%,P<0.01];3)实验组的ADM2血浆含量和收缩压呈显著负相关(r=-0.822,P<0.05)。结论ADM2作为一种心血管的活性肽,在血压调节和心肌保护过程中具有重要的意义。Background Adrenomedullin-2 （ADM2） is a novel peptide of calcitonin （CT）/calcitonin generelated peptide （CGRP） super family, which was reported by Japanese scholar in February 2004. In November 2003, Roh et al of Stanford University discovered intermedin （IMD）. They were regarded as identical substance because of their same nucleotid and amino acid sequence. ADM, CGRP, CT andIAPP （islet amyloid poly peptide） belong to CGRP super family. Recently reserch have suggested that ADM2 was involved in cardiovascular regulation. Objective To compare the difference of adrenomedullin2（ADM2） or intermedin（IMD） content and its genetic expression in plasma and tissue between SHRs and Wistar Kyoto（WKY） rats. The relationship between ADM2 in plasma, blood pressure and heart mass/body mass （HM/BM） are explored. Methods Eight male, 11-week-old SHRs were chosen as experiment group, with 8 male WKY rats of matched age as controls. Plasma and tissue ADM2 were determined by radioimmunoassay. Myocardium and aortic mRNA expression of ADM2 were determined by RT-PCR. Blood pressure and heart function were determined by cardiac catheterization. Results Comparing with the WKY rats, plasma ADM2 （317.2±25.2 vs 211.4±15.0 ng/L, P〈0. 01）, ADM2 in myocardium （293.6±34.7 vs 200.4±21.6 ng/g, P〈0.05） and in aorta （1009.0±50. 1 vs 763.8±35.0 ng/g, P〈0.01） of SHRs were significantly increased, mRNA expression of ADM2 in tissue of SHRs were also higher than those in WKY rats（ ＋ 76% in myocardium, P〈0.05; ＋ 71.11% in aorta, P〈0.01）. Plasma ADM2 was negatively correlated with SBP （r=-0. 822, P%0.05） in SHR. Conclusion These data suggest that ADM2 as a cardiovascular active peptide may involve in regulation of blood presurre and myocardial protection.

关 键 词：肾上腺髓质素2 自发性高血压大鼠 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]