HPLC法测定益肾灵颗粒中淫羊藿苷的含量  

Determination of Icariine in Yishenling Granules by HPLC

作　　者：李玥瑛[1] 笔雪艳[1] 

机构地区：[1]黑龙江省药品检验所,哈尔滨150001

出　　处：《中国药事》2007年第11期904-905,共2页Chinese Pharmaceutical Affairs

摘　　要：建立益肾灵颗粒中淫羊藿苷的含量测定方法。采用HPLC法,色谱柱:Diamonsil(C18,流动相:乙腈-水(20∶80,用磷酸调节pH为3.5),流速:1.0mL·min-1,检测波长:270nm,柱温:30℃。淫羊藿苷在0.06032～0.7540μg之间线性关系良好,r=1.000,回收率(n=6)为97.72%,RSD=0.64%。本法作为益肾灵颗粒的含量测定方法来控制制剂质量,方法简便,重现性好。A HPLC method was establishe for determinat Diamonsil C18 column was used . The mobile phase was aceton the flow rate was 1.0mL· min^-1. The detection wavelength was ion of Icariine in Yishenling Granules. A itrile-H20 （20 = 80 HaPO4 pH=3.5） and 270nm. The was a good line relationship （r =1. 000） . When the content of icariine was 0. 0632-0. 7540μg. The average recovery was 97.72%and RSD was 0. 64%. This method is simple and reproductive. It can be used for quality control of the preparation.

关 键 词：益肾灵颗粒 淫羊藿苷 HPLC 

分 类 号：R927.2[医药卫生—药学]