重组人5型腺病毒对B16黑色素瘤生长的抑制作用  

Anti-Tumor Effect of a Replication-Selective Oncolytic Adenovirus(H101) on Mice Bearing B16 Melanoma

作　　者：葛盛芳[1] 孟夏[1] 赵毅[1] 叶迅[1] 梁旻 陈红专[1] 胡放 

机构地区：[1]上海交通大学医学院,上海200025 [2]上海三维生物技术有限公司,上海201206

出　　处：《中国药学杂志》2007年第21期1633-1635,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30371614);国家科技部创新药物和中药现代化863重大专项课题资助项目(2002AA2Z3304)

摘　　要：目的研究瘤内直接注射重组人5型腺病毒H101对B16黑色素瘤生长以及对手术后再次接种B16黑色素瘤细胞后肿瘤生长的影响。方法建立小鼠B16黑色素瘤动物模型。瘤内直接注射H101腺病毒,测量肿瘤体积,切除肿瘤,再次接种B16黑色素瘤细胞,观察肿瘤生长情况。结果细胞接种8 d后,全部小鼠均长出肿瘤。H101注射组肿瘤的体积在给药后3,6,9 d,都显著小于对照组。再次接种B16黑色素瘤细胞后,H101注射组肿瘤的生长速度也显著小于对照组。结论瘤内直接注射H101对B16黑色素瘤生长具有抑制作用,对手术后再次接种的肿瘤生长也具有抑制作用。OBJECTIVE To study the inhibition effects of direct intratumor injection of H101 on B16 melanoma in C57/BL6 mice and syngenetic mice rechallenged with parental cells. METHODS C57/BL6 mice bearing B16 melanoma were established by subcutaneous seeding B16 cell suspension at a dose of 2 × 10^6 cells per mouse. H101 was injected directly into the tumor in three continuous days at a dose of 5 × 10^6 pfu · mm-3 · d^-1. The tumors were surgically excised 7 days after the injection of H101. At 14 days after surgery, all animals were rechallenged on the opposite flank with parental B16 cell at a dose of 2 × 10^5 cells. The tumor size was calculated every day to observe the antitumor effect. RESULTS The average tumor volume in the H101 injection group was signifi- cantly smaller than that in control group, not only at D3, D6 and D9, but also at every day for the surgery mice rechallenged with parental cells. CONCLUSION The direct injection of H101 showed potent antitumor effect on B16 melanoma.

关 键 词：黑色素瘤 重组人5型腺病毒H101 基因治疗 

分 类 号：R965[医药卫生—药理学]