安祖花组织培养研究  

Study on Tissue Culture of Anthurium Andraeanum Lind

作　　者：贾永芳[1] 马玉坤[2] 郭余龙[3] 李名扬[3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]甘肃农业大学生命科学技术学院,兰州730070 [3]西南大学花卉研究所,重庆400716

出　　处：《河南师范大学学报（自然科学版）》2007年第1期164-166,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家农业综合开发科技示范项目(2001108)

摘　　要：安祖花外植体的离体培养显示,在MS+2,4-D 0.1 mg.L-1+NAA0.2 mg.L-1+BA1.0 mg.L-1的培养基愈伤组织诱导率最高,达93.8%.40 d后,将愈伤组织接于MS+BA1.0 mg.L-1的培养基上,45 d后出现芽的分化.再生芽易于生根,将小芽接于MS培养基上壮苗30 d,再接于1/2MS培养基上,30 d后形成完整小植株,移栽成活率达95%以上.High yields of viable cultures of Anthuriurn Andraeanum Lind were obtaind with the use of small explants. The result showed that the MS ＋2,4-D0.1 mg · L^-1 ＋NAA0.2 mg· L^-1 ＋BA1.0 mg· L^-1 medium led to the highest callus inducing rate,which reached 93.8M. After 40 days, the ealli were transferred to the mediums of MS＋BA1.0 mg · L^-1 , buds appeared after another 45days. Root was easily formed, buds were transferred to the MS medium to strengthen them for 30d, then these buds were transferred to 1/2MS medium,and the plantlet formed after 1 month. The survival rate was more than 95%.

关 键 词：安祖花 组织培养 激素 

分 类 号：Q813.12[生物学—生物工程]