骆驼蓬总碱药理作用的研究  

STUDIES ON THE PHARMACOLOGICAL ACTION OF THE TOTAL ALKALOID OF PEGANUM HARMALA

作　　者：潘启超[1] 杨小平[1] 李春杰 胡海棠[1] 利国威[1] 曹俊 许招懂 谢冰芬[1] 潘伟光[1] 

机构地区：[1]中山医科大学肿瘤研究所

出　　处：《中山医科大学学报》1997年第3期165-167,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家自然科学基金

摘　　要：从骆驼蓬（ＰｅｇａｎｕｍｈａｒｍａｌａＬ．）种子中提取骆驼蓬总碱（ｔｏｔａｌａｌｋａｌｏｉｄｏｆｈａｒｍａｌａ，ＴＡＨ）。实验证明ＴＡＨ对６种体外培养人癌细胞有强力细胞毒作用，对３种杂种鼠移植性肿瘤和３种人癌裸鼠移植物有明显抑瘤作用，与顺铂或阿霉素有协同作用。用电子显微镜观察ＴＡＨ对肿瘤细胞超微结构影响；用显微荧光光度法分析对细胞周期的影响；用同位素掺入法测定对肿瘤细胞大分子合成的影响：用自建高效液相法进行骆驼蓬碱代谢动力学研究；毒性研究表明长期大剂量ＴＡＨ可引起可逆性肾毒性。The total alkaloid of Harmala (TAH)was isolated from the seeds of peganum harmala L. The antitumor activities of TAH was studied in vitro and in vivo.The TAH was highly cytotoxic to 6 human cancer cell lines. The growth of 3 transplantable tumors in mice and xenograft of 3 human cancers in nude mice was significantly inhibited by TAH. Synergistic antitumor effects were observed when TAH was combined with cisplatin (DDP) or adriamycin. Ultrastructural changes of various tumors after treatment of TAH were studied by electron microscope.Microfluorometric method was also used to study the effect of TAH on the cell cycle.The effects of TAH on the biosynthesis of DNA,RNA and protein in human cell lines were studied by incorporation of 3H TdR、 3H UR and 3H leu. The pharmacokinetics of harmaline in rabbits and mice were studied using a HPLC method established by us. The long term toxicity test showed that use high dose of TAH for long time can produce reversible nephrotoxicity.

关 键 词：哈马灵 哈尔明 抗肿瘤药 药理学 骆驼蓬总碱 

分 类 号：R979.1[医药卫生—药品] R285.5[医药卫生—药学]