骨髓动员外周血干细胞移植治疗严重肢体缺血的临床应用  

Autologous transplantation of peripheral blood stem cell in treatment of critical limb ischemia

作　　者：张鸿坤[1] 李鸣[1] 封华[1] 金炜[1] 赵海格[1] 陈旭东[1] 田路[1] 

机构地区：[1]浙江大学医学院附属第一医院血管外科,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2007年第4期360-363,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助(30300335)

摘　　要：目的:探讨外周血干细胞移植改善严重缺血肢体的作用。方法:15例下肢严重缺血的患者,经骨髓动员后,CS-3000Plus机器采集外周血干细胞,直接移植入缺血肢体中,共移植17人次。结果:采用骨髓动员外周血干细胞分离并移植治疗临床上严重肢体缺血的患者,术后一年,疼痛指数由术前5.0降到术后0,踝肱指数(ABI)由术前0.30提高到术后0.46;6例溃疡5例愈合;无痛行走距离由术前0.15 km提高到术后0.72 km;最大行走距离由术前0.96 km提高到术后2.13 km。结论:骨髓动员外周血干细胞移植能促进缺血肢体新生血管形成,有效改善缺血肢体的疼痛和步行距离,并促进溃疡愈合。Objective： To apply autologous transplantation of peripheral blood stem cells in the treatment of critical limb ischemia. Methods： Fifteen patients with critical lower limb ischemia were recruited in this study. After bone marrow mobilization, peripheral blood stem cells were collected using CS-3000 Plus device,and transplanted directly into the ischemic limb. In total 17 times of transplantation were performed. Results： One year after implanted peripheral blood stem cells,the pain scale decreased from 5 to 0,the ankle-brachial pressure index （ABI） increased from 0.30 to 0.46,the pain-free walking distance and maximal walking distance increased from 0.15 to 0. 72 km and 0.96 to 2. 13 km separately,from a total of 6 of 15 limb ulcers of transplanted patients 5 were healed after cell transplantation. Conclusion： Autologous peripheral blood stem cell transplantation is able to induce functional angiogenesis in ischemic limb, which can significantly reduce rest pain,increase walk distance,and promote ulcer healing.

关 键 词：干细胞 缺血 四肢/血液供给 干细胞移植 骨髓细胞 

分 类 号：R457.1[医药卫生—治疗学]