Protective effects of medical ozone combined with traditional Chinese medicine against chemically-induced hepatic injury in dogs  

作　　者：Li-Jie Li Yun-Gao Yang Zhi-Ling Zhang Sui-Feng Nie Ze Li Feng Li He-Yu Hua Yan-Jun Hu Hong-Shuan Zhang Ya-Bing Guo 

机构地区：[1]No. 425 Central Hospital ofPLA, Sanya 572008, Hainan Province, China [2]Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China [3]Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China [4]Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China

出　　处：《World Journal of Gastroenterology》2007年第45期5989-5994,共6页世界胃肠病学杂志（英文版）

基　　金：the Superintendent Foundation of Nanfang Hospital in Guangzhou, Guangdong Province, No. 20050012, ;the Foundation of Health Bureau of Guangdong Province, No. 2006388 ;the Foundation for Studies of the Science and Technology Bureau of Shenzhen, Guangdong Province, No. 200204176

摘　　要：AIM： To investigate the protective effect of medical ozone （O3） combined with Traditional Chinese Medicine （TCM） Yigan Fuzheng Paidu Capsules （YC） against carbon tetrachloride （CCh）-induced hepatic injury in dogs. METHODS： Thirty healthy dogs were divided randomly into five groups （n = 6 in each group）, namely control, oleanolic acid tablet （OAT）, O3, YC and O3 ＋ YC, given either no particular pre-treatment, oral OAT, medical ozone rectal insulfflation every other day, oral YC, or oral YC plus medical ozone rectal insulfflation every other day, respectively, for 30 consecutive days. After pre-treatment, acute hepatic injury was induced in all dogs with a single-dose intraperitoneal injection of CCh. General condition and survival time were recorded. The biochemical and hematological indexes of alanine aminotransferase （ALT）, aspartate aminotransferase/ alanine aminotransferase （AST/ALT）, serum total bUirubin （TBIL）, prothrombin time （PT）, blood ammonia （AMMO）, and blood urea nitrogen （BUN） were measured after CCh injection. Hepatic pathological changes were also observed.RESULTS： Compared to the other four groups, the changes of group O3 ＋ YC dogs＇ general conditions （motoricity, mental state, eating, urination and defecation） could be better controlled. In group O3 ＋ YC the survival rates were higher （P 〈 0.05 vs group control）. AST/ALT values were kept within a normal level in group O3 ＋ YC. Hepatic histopathology showed that hepatic injury in group O3 ＋ YC was less serious than those in the other four groups.CONCLUSION： Medical ozone combined with TCM YC could exert a protective effect on acute liver injury induced by CCI4,AIM: To investigate the protective effect of medical ozone (O3) combined with Traditional Chinese Medicine (TCM) Yigan Fuzheng Paidu Capsules (YC) against carbon tetrachloride (CCl4)-induced hepatic injury in dogs. METHODS: Thirty healthy dogs were divided randomly into fi ve groups (n = 6 in each group), namely control, oleanolic acid tablet (OAT), O3, YC and O3 + YC, given either no particular pre-treatment, oral OAT, medical ozone rectal insulfflation every other day, oral YC, or oral YC plus medical ozone rectal insulfflation every other day, respectively, for 30 consecutive days. After pre-treatment, acute hepatic injury was induced in all dogs with a single-dose intraperitoneal injection of CCl4. General condition and survival time were recorded. The biochemical and hematological indexes of alanine aminotransferase (ALT), aspartate aminotransferase/ alanine aminotransferase (AST/ALT), serum total bilirubin (TBIL), prothrombin time (PT), blood ammonia (AMMO), and blood urea nitrogen (BUN) were measured after CCl4 injection. Hepatic pathological changes were also observed.RESULTS: Compared to the other four groups, the changes of group O3 + YC dogs’ general conditions (motoricity, mental state, eating, urination and defecation) could be better controlled. In group O3 + YC the survival rates were higher (P < 0.05 vs group control). AST/ALT values were kept within a normal level in group O3 + YC. Hepatic histopathology showed that hepatic injury in group O3 + YC was less serious than those in the other four groups. CONCLUSION: Medical ozone combined with TCM YC could exert a protective effect on acute liver injury induced by CCl4.

关 键 词：Carbon tetrachloride OZONE Traditional Chinese Medicine 

分 类 号：R2[医药卫生—中医学]