表没食子儿茶素没食子酸酯抗肿瘤侵袭转移相关机制的探讨  

Effect of epigallocatechin-3-gallate on the invasiveness of hepatocarcinoma cells in vitro

作　　者：鲁藜[1] 刘惠敏[1] 汤为学[2] 

机构地区：[1]杭州市第六人民医院,310014 [2]重庆医科大学病理生理研究室

出　　处：《中华肝脏病杂志》2007年第11期825-827,共3页Chinese Journal of Hepatology

摘　　要：目的观察茶多酚的有效成分表没食子儿茶素没食子酸酯（EGCG）对人肝癌细胞侵袭转移的影响及探讨其相关机制。方法用免疫细胞化学检测黏蛋白1的表达、人工重组基底膜侵袭小室及明胶酶谱法观察其对人肝癌细胞株HepG2细胞侵袭转移的影响。结果EGCG能抑制HepG2细胞黏蛋白1的表达，对照组HepG2强阳性细胞数为15个，而EGCG组的强阳性细胞数为1个，差异有统计学意义；EGCG能抑制侵袭基底膜的能力，对照组的侵袭细胞数为（53．2±12．6）个，而EGCG组侵袭细胞数为（8．0±5．1）个，差异有统计学意义。EGCG能抑制MMP-2、MMP-9的分泌。结论EGCG具有明显的抗癌侵袭和转移的作用，其机制可能与黏蛋白1的降低有关。Objective The effect of EGCG on the invasion of HepG2 cells in vitro was studied. Methods The expressions of MUC1 in HepG2 cells before and after they were treated with EGCG were studied with immunohistochemistry. The effects of EGCG on the invasion of HepG2 cells were evaluated using Transwell chambers attached with polycarbonate fdters and reconstituted basement membranes （Matrigel）. Gelatin zymography was performed to detect the secretion of MMP-2 and MMP-9. Results EGCG reduced the expression of MUC1, suppressed significantly the invasion of tumor cells into the basement membranes （P〈0.01） and reduced the secretion of MMP-2, MMP-9. Conclusion EGCG has anti-invasion effects on HepG2 cells.

关 键 词：癌 肝细胞 肿瘤细胞 培养的 黏蛋白类 肿瘤侵袭 表没食子儿茶素没食子 酸酯 

分 类 号：R686[医药卫生—骨科学]