Targeted gene disruption by use of a group 11 intron （targetron） vector in Clostridium acetobutylicum  

作　　者：Lijun Shao Shiyuan Hu Yi Yang Yang Gu Jun Chen Yunliu Yang Weihong Jiang ShengYang 

机构地区：[1]Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology [2]Research Center of Industrial Biotechnology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China [3]Huzhou Research Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Huzhou 313000, China [4]Graduate School of the Chinese Academy of Sciences, Beijing 100080, China

出　　处：《Cell Research》2007年第11期963-965,共3页细胞研究（英文版）

摘　　要：Dear Editor： Clostridium acetobutylicum, a gram-positive, anaerobic, spore-forming bacterium, is capable of using a wide variety of carbon sources to produce acetone, butanol and ethanol. To improve solvent productivity of C. acetobutylicum, metabolic engineering is considered as a useful tool in developing strains with industrially desirable character-istics. However, to date, there are few useful methods for genetic manipulation of C. acetobutylicum, especially for gene disruption. To our knowledge, two types of vectors, including non-replicative and replicative integrative plasmids, have been developed for gene-inactivation in C. acetobutylicum. By using non-replicative integrative plasmids, buk and solR genes of C. acetobutylicum were inactivated [1,2]. However, due to their low frequencies of transformation and recombination, the non-replicative integrative plasmids are usually transformed at less than 1 integrative transformant per mg plasmid DNA. To obtain the integrative mutant, it may require higher transformation frequencies up to 10^5, but the typical transformation fre-quencies were reported at 10^3 [3].

关 键 词：11内含子 梭菌属 基因表达 细胞研究 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]