HPLC法测定栽培河套大黄中土大黄苷的含量  

HPLC determination of rhaponticin in Rheum hotaoense C.Y.Cheng et C.T.Kao

作　　者：逯海龙 韩义欣 董文静 

机构地区：[1]宁夏药品检验所,银川750001

出　　处：《药物分析杂志》2007年第11期1809-1811,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：宁夏科技厅自然科技基金;NZ0649

摘　　要：目的:建立 HPLC 法测定栽培河套大黄中土大黄苷的含量。方法:采用 ZORBAX Eclipse XDB—C_(18)(150 mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-水(35:5:60)为流动相,流速:1.0 mL·min^(-1),检测波长为320 nm,采用外标法定量测定。结果:土大黄苷进样量在0.206～2.06μg范围内与峰面积呈良好的线性关系(r=0.9998);平均回收率(n=6)为99.31%,RSD 为0.82%。结论:本方法操作简便,结果准确可靠,适用于栽培河套大黄中土大黄苷的含量。Objective：To establish an HPLC method for the content determination of rhaponticin in Rheum ho- taoense C. Y. Cheng et C. T. Kao. Method： A ZORBAX Eclipse XDB - Cis column （ 250 mm × 4. 6 mm, 5 μm） was used as the analytical column and mobile phase was methanol -acetonitrile -water（35： 5： 60）at a flow rate of 1.0 mL·min^-1 and the UV detection wavelength was set at 320 nm. Results： The linear range was 0. 206 - 2. 06μg（ r = 0. 9998 ） for 1 rhaponticin ;The average recovery （ n = 6 ） of rhaponticin were 99.31%, RSD = 0. 82%. Conclusion： This method is simple, rapid and reliable for determination of rhaponticin in Rheum hotaoense.

关 键 词：河套大黄 土大黄苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]