浊度法测定注射用硫酸奈替米星的含量  

Turbidimetric determination of Netilmicin Sulfate for injection

作　　者：易大为[1] 潘强[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《药物分析杂志》2007年第11期1816-1819,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立浊度法测定注射用硫酸奈替米星的含量并进行方法学验证。方法:以金黄色葡萄球菌制作菌悬液,接种于抗生素检定Ⅲ号培养基中,取9 mL 培养液和1 mL 不同浓度的硫酸奈替米星置比色管中,37℃培养4 h 左右,530 nm 在线测定。结果:培养物浊度与奈替米星浓度的 log 值在0.6～1.6 u·mL^(-1)之间呈线性关系(r>0.99);测得奈替米星的平均回收率为100.7%(RSD=3.4%)。结论:该方法准确、可靠、灵敏度高,耗时短。Objective ：To establish turbidimetric method with determination Netilmicin Sulfate for injection. Method： Inoculate medium IU with a suspension of Staphylococcus aureus, Distribute 9 mL of the inoculated medium and 1 mL of the Netilmiein Sulfate series of doses＇ solution into test - tubes, determine on - linely on 530 nm after an incubation period of about 4 h at 37 ℃. Result： Linearity of the dose - response relationship about the turbidity and the antibiotic doses＇ log was between 0. 6 - 1.6 u · mL^-1 （r 〉0. 99） ;The average recovery was 100. 7% （ RSD = 3.4% ）. Conclusion ：The method is accurate, reliable, sensitive and quick.

关 键 词：浊度法 硫酸奈替米星 金黄色葡萄球菌 

分 类 号：R917[医药卫生—药物分析学]