半微柱高效液相色谱-电化学法检测和厚朴酚与厚朴酚  

Determination of Honokiol and Magnolol by semi-micro HPLC with electrochemical detection

作　　者：王大力[1] 王清珊[1] 金东日[1] 

机构地区：[1]延边大学理学院化学系,133002

出　　处：《药物分析杂志》2007年第11期1820-1823,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：白中笹川医学奖学金研究者资助项目(2003);延边大学校项(2003-35)

摘　　要：目的:用半微柱高效液相色谱-电化学检测技术建立了高灵敏、选择性的和厚朴酚与厚朴酚的检测方法。方法:色谱柱为 CAPCELL PAK C18 UG120 S-3 μm(1.0 mm×150 mm),流动相为含有0.5%磷酸的甲醇-水(64:36或60:40,v/v),极化电位为+0.8 V(vs.Ag/AgCl 参比电极)。结果:和厚朴酚与厚朴酚的浓度与峰电流的线性关系良好,线性范围均为2.5fmol～7.5 pmol,检出限均为0.5 fmol。在不同进样量(50 fmol、1 pmol、5 pmol)下,和厚朴酚与厚朴酚的 RSD 均小于2.3%(n=10)。结论:该法可应用于血浆和中药制剂中和厚朴酚与厚朴酚的测定。Objeetive：A highly sensitive and selective method was developed for the determination of honokiol and magnolol HPLC- ECD using a microbore column. Method： HPLC conditions were column CAPCELL PAK C18 UG120 S - 3μm（ 1.0 mm × 150 mm） ,methanol - water（64：36 and 60：40 ,v/v） containing 0. 5 % phosphoric acid and polarization potential set at ＋ 0. 8 V（ vs. Ag/AgCl）. Result：Peak heights for honokiol and magnolol were found to be linearly related to the amount of each honokiol and magnolol injected from 2. 5 fmol -7.5 pmol. Both of the detection limit（ signal -noise ratio,S/N = 3）of magnolol and honokiol were 0. 5 fmol. The relative standard deviations（RSD,n = 10）were less than 2.3 % at 50 fmol,1 pmol and 5 pmol for honokiol and magnolol. Conclusion： The proposed method was applied to determine honokiol and magnolol in traditional Chinese herbal medicines and human plasma sample.

关 键 词：半微柱-HPLC 和厚朴酚 厚朴酚 

分 类 号：R917[医药卫生—药物分析学]