桦褐孔菌提取物诱导人胃癌BGC-823细胞凋亡的研究  

Apoptosis of Human Gastric Cell Line BGC-823 Induced by Extracts from Inonotus Obliquus

作　　者：钟秀宏[1] 杨淑艳[1] 孙东植[2] 张以忠[1] 郑中华[1] 赵丽薇[1] 

机构地区：[1]吉林医药学院病理教研室,吉林市132013 [2]延边大学基础医学院病理与法医教研部,延吉市133000

出　　处：《中国药房》2007年第34期2661-2663,共3页China Pharmacy

摘　　要：目的:探讨桦褐孔菌提取物对肿瘤细胞周期的影响及其抗癌的作用机制。方法:将不同浓度(20、40、80mg·L-1)桦褐孔菌提取物作用于人胃癌BGC-823细胞48h,用碘化丙啶染色法检测凋亡细胞和细胞周期分布。采用免疫细胞化学染色法检测药物处理前、后Ki-67抗原的表达。结果:3种浓度桦褐孔菌提取物处理48h后细胞凋亡率分别为11.57%、20.97%、31.22%,阻滞细胞周期于S期,并使Ki-67阳性表达率下降,且具有浓度依赖性。结论:桦褐孔菌提取物可抑制人胃癌BGC-823细胞的生长,细胞主要阻滞于S期,并能诱导部分细胞凋亡。OBJECTIVE： To investigate the possible mechanism of anticancer activity of the extracts from Inonotus obliquus and its association with the phase of cell cycle, METHODS： FCM was used to detect cell cycle distribution of human gastric carcinoma BGC- 823 cells that treated with extracts from Inonotus obliquus at different concentrations （20, 40, and 80 mg· L^-1） for 48h and their apoptotic rate, marked by PI. Furthermore, the protein expressions of Ki- 67 were detected by immunocytochemical staining method before and after exposure to extracts from Inonotus obliquus. RESULTS： After being treated by extracts from Inonotus obliquus （20, 40, and 80mg · L^-1） for 48h, the percentage of apoptosis cells were 11.57%, 20.97%, 31.22%, respectively and the human gastric carcinoma BGC-823 cells were inhibited and arrested in S phase. The protein expression of Ki- 67 was decreased dose- dependently. CONCLUSION： The extracts from Inonotus obliquus can inhibit the growth of human gastric carcinoma BGC - 823 cells much as in S phase and induce apoptosis of BGC-823 cells.

关 键 词：桦褐孔菌提取物 胃癌细胞BGC-823 凋亡 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]