Phenotypic characterization of a rice mutant Oryza sativa extraordinary glume 1(Oseg 1)and its genetic analysis  

水稻颖壳异常发育突变体Oryza sativa extraordinary glume 1(Oseg 1)的遗传与基因初定位分析(英文)

作　　者：王红梅 储黄伟 刘海生 李晓星 杨功达 张大兵 雍克岚 

机构地区：[1]School of Life Sciences,Shanghai University [2]SJTU-SIBS-PSU Joint Center for Life Sciences,School of Life Science and Biotechnology,Shanghai Jiaotong University

出　　处：《Journal of Shanghai University(English Edition)》2007年第6期619-624,共6页上海大学学报（英文版）

基　　金：Project supported by the National Key Basic Research Development Program of the Ministry of Science and Technology of China(Grant Nos.2001CB109002,2005CB120802);the National High-Techhology Research and Development Program of China(Grant No.2005AA2710330);the Science Foundation of Shanghai Municipal Commission of Science and Technology(Grant Nos.03JC14061,03DJ14016);the Program for New Century Excellent Talents in University(Grant No.NCET-040403);the Shuguang Plan of Shanghai Education Development Foundation(Grant No.04SG15)

摘　　要：A rice mutant with Yaponica 9522 cultivar background Oryza sativa extraordinary glume 1 （Oseg 1） was identified from the M2 mutant pool mutagenized by ^60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F2 population was constructed by a cross between Oseg 1 （Japonica） and Guangluai4 （Indica）. Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene.A rice mutant with Yaponica 9522 cultivar background Oryza sativa extraordinary glume 1 （Oseg 1） was identified from the M2 mutant pool mutagenized by ^60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F2 population was constructed by a cross between Oseg 1 （Japonica） and Guangluai4 （Indica）. Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene.

关 键 词：rice （Oryza sativa L.） GLUME OsEG1 spikelet meristem primary mapping. 

分 类 号：S511[农业科学—作物学]