人精子保存于HT卵黄缓冲液前后的可分析染色体获得率与自然畸变率比较  

Comparison between the rates of analysible chromosome and nature distortion of sperms before/after being stored in HT yolk buffer

作　　者：李运星[1] 马天根[1] 唐瑟[2] 

机构地区：[1]四川省计划生育科学研究所 [2]华西医科大学附属第二医院

出　　处：《男性学杂志》1997年第2期79-81,共3页

基　　金：国家自然科学基金;国家计生委;四川省科委共同资助

摘　　要：将人精液分为两组，一组直接用于制备精子单倍染色体，一组保存于４～８℃的ＨＴ卵黄缓冲液中４８小时，再用于制备精子单倍染色体。比较两组的可分析染色体获得率与自然畸变率，了解ＨＴ卵黄缓冲液是否会造成自然畸变率的上升和可分析染色体获得率的下降。对３５份样品试验与观察得到两组间可分析染色体获得率比较结果，Ｘ２＝０．１５９，Ｐ＞０．０５；自然畸变率两组比较结果，Ｘ２＝０．０７４，Ｐ＞０．０５。结果表明两组间在这两个率比较中无统计学差异，ＨＴ卵黄缓冲液可应用于精子在冷环境中短期贮藏，并不具有致染色体突变的效应。Human semens were separated into two groups. The one group semen was used to preparing the sperm haploid chromosome directly. Another group semen were stored in HT yolk buffer for 48 hours at 4～8℃,then the sperms from the semen were resuscited at 37℃ for preparing the sperm haploid chromosome. During 35 times repeating experiments and comparing the rates of analysible sperm chromosome and nature distortion of the chromosome between the two groups, there are not any statistical differences between the two groups' rates ( P >0.05). The HT yolk buffer does not have any effect of making the chromosome distortion. The HT yolk buffer can be used in storing human sperm at 4～8℃ for a short term for clinic examination and some research purpose in andrology.

关 键 词：HT卵黄缓冲液 精子 单倍染色体 自然畸变 

分 类 号：R698.2[医药卫生—泌尿科学]