牙鲆Follistatin基因启动子克隆与分析  

Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene.

作　　者：刘庆华[1] 谭训刚[1] 徐永立[1] 张培军[1] 张玉青[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室

出　　处：《浙江大学学报（农业与生命科学版）》2007年第6期616-620,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家高技术研究发展计划"863"资助项目(2004AA628110;20060110A4013);国家重点基础研究发展计划"973"资助项目(2004CB117402)

摘　　要：为研究海水鱼中Follsitatin基因的组织表达特异性,利用PCR方法获得牙鲆Follistatin基因启动子并进行序列分析,用RT-PCR和显微注射方法研究Follistatin基因与肌肉发育的关系.结果表明,所获得的启动子含有USF、E47、MyoD、NF-Y等多种转录因子结合位点;在牙鲆成体组织中,Follistatin基因在体肾、肠、心脏、头肾、脾中有表达,而在肌肉和肝脏中没有表达;显微注射证明Follistatin基因参与了肌肉的早期发育.In order to study the tissue expression character of Follistatin gene in marine fish, the promoter of Paralichthys olivaceus Follistatin gene was isolated by PCR, and the sequence was analyzed; the function of Follistatin gene on the development of muscle was studied by RT-PCR and microinjection. The results indicated that the promoter had many transcription factor binding sites such as USF, E47, MyoD and NF-Y, et al ; in Paralichthys olivaceus adult tissues, Follistatin expressed in body kidney, intestine, heart, head kidney and spleen, while did not express in muscle and liver; microinjection revealed that Follistatin gene played a role in the early development of muscle.

关 键 词：牙鲆(Paralichthys olivaceus) 卵泡抑素(Follistatin) 启动子 克隆 肌肉发育 

分 类 号：Q344[生物学—遗传学]