牙鲆Follistatin cDNA的克隆与序列分析  

Cloning and sequence analysis of Paralichthys olivaceus Follistatin cDNA.

作　　者：刘庆华[1] 谭训刚[1] 徐永立[1] 张培军[1] 徐芃[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室

出　　处：《浙江大学学报（农业与生命科学版）》2007年第6期621-625,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家高技术研究发展计划"863"资助项目(2004AA628110;20060110A4013);国家重点基础研究发展计划"973"资助项目(2004CB117402)

摘　　要：为研究海水鱼中Follistatin基因的序列特征,从受精后67 h的牙鲆受精卵中克隆获得牙鲆Follistatin基因cDNA序列,并对其进行序列分析.结果表明,在牙鲆中并没有发现Follistatin基因的可变式剪切现象;牙鲆Follistatin基因cDNA序列全长963 bp.序列分析结果显示:牙鲆与Takifugurubripes的亲缘关系最近.Follistatin蛋白中高度保守的半胱氨酸和甘氨酸残基的生物学功能可能是维持蛋白空间结构的稳定.To study the DNA sequence features of Follistatin gene in marine fish, Paralichthys olivaceus Follistatin cDNA was isolated and analyzed from Paralichthys olivaceus embryos 67 h after fertilization. The results indicated that there was no evidence for alternative splicing of Follistain in Paralichthys olivaceus. The length of Paralichthys olivaceus Follistatin cDNA was 963 bp. Sequence analysis revealed that Paralichthys olivaceus Follistatin was more closely related to Takifugu rubripes. The biological function of the highly conserved cysteine and glycin in Paralichthys olivaceus Follistatin protein might be maintaining the stabilization of protein structure.

关 键 词：牙鲆(Paralichthys olivaceus) 卵泡抑素(Follistatin) 序列分析 

分 类 号：Q781[生物学—分子生物学]