焦炉作业工人芳烃受体单核苷酸多态性及其单体型与染色体损伤的关系  

Association between aryl hydrocarbon receptor gene polymorphisms and chromosomal damage in coke-oven workers

作　　者：宾萍[1] 冷曙光[1] 梁学邈[2] 程娟[1] 戴宇飞[1] 李海山[1] 段化伟[1] 牛勇[1] 张林媛[1] 刘清君[1] 林涵[2] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]鞍山钢铁公司劳动卫生研究所

出　　处：《卫生研究》2007年第6期664-666,670,共4页Journal of Hygiene Research

基　　金：国家973计划资助项目(No2002CB512903);国家自然科学基金资助项目(No30400348和30625031)

摘　　要：目的研究芳烃受体(AHR)基因多态性与焦炉作业工人外周血淋巴细胞染色体损伤易感性的关系。方法选取89名焦炉作业工人(暴露组)和60名无职业性多环芳烃及射线暴露的工人(对照组)为研究对象。测定尿中1-羟基芘反映多环芳烃暴露内剂量,用双核淋巴细胞微核评价个体外周血淋巴细胞染色体损伤,采用聚合酶链反应-限制性片段长度多态性方法分析AHR基因rs6960165及rs2282885位点多态性,利用PHASE2.1软件经Bayesian法计算单体型。用协方差统计方法分析不同基因型及单体型对与染色体损伤水平的关系。结果暴露组尿1-羟基芘水平显著高于对照组(P<0.01),暴露组外周血淋巴细胞微核率显著高于对照组(P<0.01)。在校正年龄和自然对数转换后的1-羟基芘后,暴露组AHR基因rs6960165位点多态性与微核率有关联(P=0.014),未发现对照组AHR基因rs6960165位点多态性与微核率有关联(P=0.586);未发现暴露组和对照组的AHR基因rs2282885位点多态性与微核率有关联(P=0.308和P=0.415)暴露组AHR基因不同单体型对与微核率有关联(P=0.007),未发现对照组AHR基因不同单体型对与微核率有关联(P=0.768)。结论AHR基因rs6960165位点多态性及AHR基因单体型是影响焦炉作业工人外周血淋巴细胞染色体损伤水平易感性的可能因素之一。Objective To investigate the association of single nucleotide polymorphisms（SNPs） or haplotypes of aryl hydrocarbon receptor（AHR） gene and chromosomal damage in peripheral blood lymphocytes among coke-oven workers. Methods Eighty-nine coke-oven workers exposed to a high level of polycyclic aromatic hydrocarbons （PAHs） and sixty non-exposed workers were selected as the study subjects. Urinary 1-hydroxypyrene （1-OHPyr） levels were measured as the internal dose of PAHs exposure. The chromosomal damage in peripheral lymphocyte was measured by the cytokinesis-block micronucleus（CBMN） assay. Two SNPs in AHR gene, including rs6960165, rs2282885 were detected by PCR-RFLP. The AHR haplotypes were estimated by Bayesian statistical method with the software of PHASE Version 2.1. The associations between SNPs or haplotypes pairs and CBMN were assessed by analysis of covariance in the coke-oven workers and nonexposed workers. Results The level of 1-OHPyr among coke-oven workers was significantly higher than that among nonexposed workers （P 〈 0.01 ）. The CBMN among coke-oven workers was significantly higher than that among non-exposed workers （ P 〈 0.01 ）. After adjusting the age and the level of 1-OHPyr, the different SNPs of AHR gene rs6960165 in coke-oven workers were related to the CBMN frequencies （ P = 0.014）, but no association between the different SNPs of AHR gene rs2282885 and the rates of CBMN was observed in coke-oven workers （ P = 0.586）, either in the controls（ P = 0.308 and P = 0.415, respectively）, the haplotypes in coke-oven workers were significantly related to the rates of CBMN （ P = 0.007） , while there was no significant association in non-exposed workers （ P = 0.768） . Conclusion Our results suggested that SNPs rs6960165or haplotypes of AHR were associated with the CBMN frequencies in coke-oven workers.

关 键 词：芳烃受体 微核试验 多环芳烃 染色体损伤 基因 环境毒理 

分 类 号：R135.1[医药卫生—劳动卫生] R994.6[医药卫生—公共卫生与预防医学]