动脉粥样硬化氧化应激机制的探讨  

Study of Mechanism of Oxidative Stress in Atherosclerosis

作　　者：张娟[1] 孙明[1] 周宏研[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008

出　　处：《医学临床研究》2007年第7期1148-1150,共3页Journal of Clinical Research

摘　　要：【目的】探讨动脉粥样硬化(AS)的氧化应激状态及其机制。【方法】日本大耳白兔20只,随机分为两组:每组10只,对照组以普通饲料喂养;模型组以高胆固醇饲料喂养;共喂养16周后,分别测定血清超氧化物歧化酶(SOD)活性,同时运用免疫组织化学染色法检测主动脉血管紧张素受体-1(AT1-R)的表达,内参比法RT-PCR定量分析p22phox mRNA在主动脉的表达。【结果】模型组血清SOD较对照组显著降低(P<0.01),模型组主动脉AT1-R及p22phox mRNA表达显著高于对照组(P<0.01)。【结论】①动脉粥样硬化时体内活性氧升高;②活性氧升高的机制可能与血管AT1-R及p22phox表达增强有关。[Objective]To study the mechanism of oxidative stress in atherosclerosis [Methods]1.Twenty rabbits were randomly divided into two groups：①Normal control group ： 10 rabbits were fed with common food twice daily;②Model group： 10 rabbits were fed with high cholesterol diet supplemented by Cholesterol 0.5 g/（kg·day）,yolk 2 g/（kg·day） and lard 2 g/（kg·day）;After 16 weeks,blood samples were taken for determination of concentration of serum superoxide dismutase（SOD）.AT1 receptor was assessed by immunohistochemistric assays.p22phox subunit mRNA in aorta was quantified with semiquantitative RT-PCR methodology.[Results]In model group,concentration of serum SOD significantly decreased（P〈0.01）as compared to those in control group.In model group,accumulation of AT1 receptors and mRNA expression of p22phox in aorta significantly increased as compared to those in control group（P〈0.01）[Conclusion]①ROS increases in atherosclerotic individuals.②The increasing of AT1 receptors and mRNA expression of p22phox in aorta may be the mechanism of oxidative stress in atherosclerosis.

关 键 词：动脉粥样硬化 氧化性应激 

分 类 号：R543.5[医药卫生—心血管疾病]