氟伐他汀对单侧输尿管梗阻大鼠肾间质纤维化的影响  

Effect of Fluvastatin to Fibrosis of Renal Interstitium in Rat's Kidney with Unilateral Ureteric Obstruction

作　　者：刘芝兰[1] 宁建平[2] 潘小群[1] 

机构地区：[1]湖南湘潭职业技术学院医学院,湖南湘潭411100 [2]中南大学湘雅医院肾内科,湖南长沙410008

出　　处：《医学临床研究》2007年第8期1277-1279,1282,共4页Journal of Clinical Research

摘　　要：【目的】观察氟伐他汀对单侧输尿管梗阻后大鼠肾间质单核细胞趋化蛋白-1(MCP-1)表达、单核/巨噬细胞浸润及肾间质纤维化程度的影响,探讨他汀类药物的抗炎作用机制及对肾脏的保护作用。【方法】72只SD雌性大鼠随机分成假手术(SOR)组、单侧输尿管梗阻术(UUO)组和单侧输尿管梗阻术(UUO)+氟伐他汀治疗组[T-UUO组,氟伐他汀20 mg/(kg.d)]。于术后d3、d7、d10、d14分别处死各组大鼠。用HE及Masson染色动态观察肾脏病理变化,免疫组织化学法测定MCP-1的表达和巨噬细胞浸润情况。【结果】UUO组肾小管-间质MCP-1与单核巨噬细胞抗原ED-1表达较SOR组增加(P<0.05);在术后各时间点,T-UUO组大鼠肾小管-间质MCP-1、ED-1的表达及肾间质胶原相对面积较UUO模型组显著减少,但仍高于SOR组(P<0.05)。【结论】氟伐他汀可通过降低MCP-1表达、抑制单核-巨噬细胞浸润,减轻肾间质纤维化而对肾脏有保护作用。[Objective]To evaluate the effect of Fluvastatin on monocyte chemoattractant protein-1（MCP- 1） and macrophages infiltration and renal interstitial fibrosis in rat, explore the mechanism of statin-genealogical drugs about antiphlogistic and protective function to Kidney. [Methods]Seventy-two female Sprague-Dawly rats were distributed casually into sham-operated-groups （SOR） and unilateral ureteric obstructive operated groups （UUO） and unilateral ureteric obstructive operation with fluvastatin treatment groups [T-UUO, Fluvastatin 20mg/（kg·d）]. Six rats of each group were killed respectivelly at 3,7,10,14 days after unilateral ureteric obstractive operation. Immunohistochemistry was used to measure level of expression of MCP-1 and macrophages infiltration. The changes of renal tissue were also observed by HE and Masson stain. [Results]The level of expression of tubulointerstitial MCP-1 and ED-1 of monocyt-macrophages antigen showed a marked increase in UUO when compared to SOR （ P〈0.05）. At every time point after operation, in T-UUO, the level of expression of tubulointerstitial MCP-1 and ED-1 and relative surface of renal interstitial collegans in rat＇s kidney showed also a marked decrease when compared to UUO. But it still showed increase when compared to SOR（ P〈0.05） . [Conclusion]Fluvastatin possesses the function for protecting kidney of rat by decreasing the level of expression of MCP-1 and inhibiting infiltration of monocyte-macrophage and reducing renal interstitial fibrosis.

关 键 词：输尿管梗阻/药物疗法 肾小球硬化症 病灶性 普伐他丁/药理学 大鼠 

分 类 号：R693.2[医药卫生—泌尿科学]