联合应用巴曲酶和依达拉奉对沙土鼠缺血再灌注后凋亡相关基因表达的影响  

Effect of Batroxobin and Edaravone Cooperation on Apoptosis-related Gene Expression After Cerebral Ischemia/Reperfusion in Gerbils

作　　者：李娟[1] 丁新生[2] 印卫兵[2] 冯美江[1] 高阳[2] 龚洁[2] 

机构地区：[1]南京医科大学第二附属医院神经内科,江苏南京210011 [2]南京医科大学第一附属医院神经内科,江苏南京210029

出　　处：《医学临床研究》2007年第8期1294-1296,共3页Journal of Clinical Research

摘　　要：【目的】探讨联合应用巴曲酶和依达拉奉与单独使用巴曲酶对沙土鼠缺血再灌注后凋亡相关基因表达的影响。【方法】阻断沙土鼠双侧颈总动脉5 min造成前脑缺血再灌注模型,并将其随机分为假手术组、对照组、巴曲酶组、巴依联合治疗组。采用免疫组织化学法检测沙土鼠缺血再灌注不同时间内海马CA1区Bcl-2和Bax表达水平。【结果】联合使用巴曲酶和依达拉奉后,Bcl-2表达明显增加,Bax表达明显降低,阳性细胞数在各时间点与巴曲酶组、对照组比较差异有显著性（P〈0.05）;Bcl-2、Bax阳性细胞数在巴曲酶组0～24 h时间点之间比较差异无统计学意义（P〉0.05）;在0～24 h时间点与48～72 h时间点比较差异有统计学意义（P〈0.05）;Bcl-2、Bax阳性细胞数在巴依联合治疗组0～48 h时间点之间比较差异无统计学意义（P〉0.05）;在0～48 h时间点与72 h时间点比较差异有统计学意义（P〈0.05）。【结论】联合应用巴曲酶和依达拉奉较单独使用巴曲酶可明显减少脑缺血再灌注后神经细胞的凋亡,发挥脑保护作用;且可延长巴曲酶治疗的时间窗;其机制可能与Bcl-2表达的增强及Bax表达的抑制有关。[Objective]To discuss the effect of Batroxobin with Edaravone or without Edaravone on apoptosis-related gene expression after cerebral ischemia/reperfusion in gerbils. [Methods]Transient forebrain isehemia was induced by 5 minutes bilateral carotid occlusion. All gerbils were randomly divided into sham operation group, control group, Batroxobin group, Batroxobin and Edaravone cooperation group. After transient forebrain ischemia in gerbils followed by reperfusion, variation of Bcl-2 and Bax expression were detected by immunohistochemical method. [Results]The expression of Bcl-2 remarkably increased in Batroxobin and Edaravone Cooperation group, whereas the expression of Bax remarkably decreased in Batroxobin and Edaravone Cooperation group. There were significant differences among sham operation group, control group, Batroxobin group, Batroxobin and Edaravone Cooperation group （ P〈0.05）. There weren＇t any significant differences among 0～24 h in Batroxobin group （ P〉0.05） ,but a significant difference between 0～24 h and 48～72 h. In Batroxobin and Edaravone Cooperation group, there weren＇t any significant differences among 0～48 h （ P〉0.05）, but a significant difference between 0～48 h and 72 h. [Conclusion]Comparison to the Batroxobin group, Batroxobin and Edaravone cooperation could significantly reduce apoptosis of neurons in hippocampal CAt territory of gerbils, then protect their brains; furthermore the cooperation prolongs the therapy time of Batroxobin, which probably induced the increasing of Bcl-2 expression and the decreasing of Bax expression.

关 键 词：再灌注损伤 基因表达 脱噬作用 蛇毒凝血酶 沙鼠亚科 

分 类 号：R619.9[医药卫生—外科学]