兔出血症病毒复制子的构建及其在RK-13细胞中的复制  

Construction of Rabbit Hemorrhagic Disease Virus Replicons and Its Replication in RK-13 Cells

作　　者：刘光清[1] 倪征[1] 云涛[1] 余斌[1] 朱金梅[2] 华炯钢[1] 陈剑平[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,杭州310021 [2]西北农林科技大学动物科技学院,杨凌712100

出　　处：《病毒学报》2007年第6期481-484,共4页Chinese Journal of Virology

基　　金：国家自然科学基金项目(30670074);浙江省自然科学基金(Y305074)

摘　　要：为研究兔出血症病毒(RHDV)的复制机制、病毒与宿主之间的相互作用以及致病机制等,创建一个安全、有效的技术平台,在前期构建的RHDV侵染性克隆基础上,将病毒的衣壳蛋白编码区删除,保留了RHDV复制必需的所有蛋白酶基因和两端的非编码区,构建了RHDV复制子。试验结果证明,将该复制子RNA导入RK13细胞中后,能够进行高水平的复制和表达。To provide an efficient and safe technology platform for studying the replication and pathogenesis mechanisms of RHDV, the interaction between the RHDV and its host cells, a replicon system of RHDV, was constructed based on the infectious cDNA clone of RHDV, in which VP60 gene encoding the capsid protein was deleted, but all the necessary protease coding regions and non-coding regions were retained. Results from RT-PCR, IFA and qRT-PCR confirmed that the replicon RNA could efficiently replicate in RK-13cells. Besides, the results also suggested that the capsid protein which is the structural protein of RHDV is necessary for maintaining the viral infectivity.

关 键 词：兔出血症病毒 复制子 衣壳蛋白 RK-13细胞 

分 类 号：S858.291[农业科学—临床兽医学]