云南昭通黄牛mtDNA D-loop区遗传变异分析  

Study on mtDNA D-loop Region Genetic Diversity in Zhaotong Cattle

作　　者：周艳[1] 陈宏[1] 贾善刚[1] 房兴堂[2] 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,江苏徐州221116

出　　处：《中国牛业科学》2007年第6期38-41,共4页China Cattle Science

基　　金：国家高技术研究发展计划(863计划)项目(编号:2006AA10Z197);国家自然科学基金项目(30471238);国家支撑计划项目(编号:2006BAD01A10-5);西北农林科技大学拔尖人支持计划项目

摘　　要：为检测云南昭通黄牛的遗传多样性，测定并分析了云南昭通黄牛线粒体D—loop区段全序列。结果显示，D—loop序列四种碱基A、T、C、G的频率分别是：32．9％、28．6％、24．8％和13．6％；共检测到48个变异位点，平均核苷酸差异（k）为22．762，核苷酸多样度（π）为2．504％，存在6个单倍型，单倍型多样度（Hd）为0．952±0．096，显示出极高的遗传多样性。检索了与昭通黄牛地理分布相近的8个黄牛品种的D—loop序列资料，统计分析发现，9个黄牛品种间的遗传距离在0．0000~0．0376之间，昭通与其他黄牛品种的遗传距离最远，提示云南黄牛有着特殊的起源地位。The complete mitochondrial D--loop sequences, 910 bp in length, in 7 individuals from Zhaotong cattle breed in Yunnan province of China were detected and analyzed. The results showed that frequency of A, T, C, G nucleotides in D--loop region were 32.9%,28.6%,24. 8% and 13.6%, respec tively. 48 variations sites were detected. Average number of nu cleotide diversity （π） was 2. 504%, and 6 haplotypes were discov ± 0. 096, indicating high genetic diversity in Zhaotong cattle pop of 8 cattle breeds whose geographical distributions draw near Zh cleotide differences （k） was 22. 762, nuered, haplotype diversity （Hd）was 0. 952 ulations. Sequence data of D--loop region aotong cattle were searched from publication. Genetic distances among 9 cattle breeds were calculated with 0. 0000~0.0376.Zhaotong cattle showed the most remote genetic relationship with orthers, indicating a special origi Yunnan province.

关 键 词：昭通黄牛 线粒体D—loop区 遗传多样性 起源 

分 类 号：S823.2[农业科学—畜牧学]