谷胱甘肽S-转移酶P1基因多态性与儿童支气管哮喘易感性  

Association between Polymorphism of Glutathione S-Transferease P1 Gene and Susceptibility in Childhood Asthma

作　　者：李海林[1] 熊晶晶[1] 黄永坤[1] 

机构地区：[1]昆明医学院第一附属医院儿科,昆明650032

出　　处：《实用儿科临床杂志》2007年第4期264-266,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨谷胱甘肽S-转移酶P1（GSTP1）基因多态性与儿童哮喘易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析检测51例哮喘患儿（病例组）和40例正常儿童（对照组）GSTP1 Ile105/Ile105、Ile105/Val105和Val105/Val105三种基因型分布频率。结果GSTP1基因的Ile/Ile、Ile/Val、Val/Val基因型分布频率在病例组分别为66.7%、27.4%、5.9%,Ile和Val等位基因的分布频率为80.4%、19.6%;而对照组Ile/Ile、Ile/Val、Val/Val基因型分布频率分别为90.0%、7.5%、2.5%,Ile和Val等位基因的分布频率为93.8%、6.2%。二组GSTP1基因型的分布频率差异显著（χ^2=6.947 P〈0.05）。病例组Ile/Val和Val/Val基因型占优势（OR=4.5,95%可信区间1.375-14.729）。二组GSTP1基因Ile和Val等位基因的分布频率差异显著（χ^2=6.751 P〈0.05）,病例组Val等位基因的分布频率高于对照组。结论GSTP1 Ile/Val、Val/Val基因型与哮喘存在相关性,Val等位基因与哮喘易感性有关,提示GSTP1基因多态性可能在哮喘遗传易感性方面有重要作用。Objective To investigate the polymorphism of glutathione S-transferease P1 gene（GSTP1） and the association between the mutation and susceptibility in childhood asthma.Methods The distribute frequency of Ile105/Ile105,Ile105/Val105 and Val105/Val105 ge-notype in GSTP1 of 51 children with asthmatic and 40 normal children were studied with polymerase chain reaction-restriction tragment length polymorphism（PCR-RFLP）.Results The frequencies of Ile/Ile Ile/Val,Val/Val genotype were 66.7%,27.4% and 5.9%,the frequencies of Ile,Val allele were 80.4% and 19.6% in the asthmatic group.But the frequencies of Ile/Ile,Ile/Val,Val/Val genotype were 90.0%,7.5% and 2.5%,the frequencies of Ile,Val allele were 93.8%,6.2% in control group.The frequencies Ile/Val,Val/Val genotype and Val allele in asthmatic group were more than that in control group.A significant difference was found in the frequency distribution of GSTP1 genotypes between two groups（χ^2=6.947 P〈0.05）.In the asthmatic group,Ile/Val,Val/Val genotypes were more common than those of control group（OR=4.5,95%CI 1.375～14.729）.Moreover,GSTP1 allele frequencies also showed significant difference between two groups（χ^2=6.751 P〈0.05）.Val allel was more common in the asthmatic group than that in control group.Conclusions There is a relationship between GSTP1 Ile/Val、Val/Val genotypes and asthma.GSTP1 gene polymorphism may play an important role in determining an individual′s susceptibility to development of asthma.

关 键 词：哮喘 谷胱甘肽S-转移酶P1 基因多态性 遗传易感性 

分 类 号：R725.6[医药卫生—儿科]