D-Limonene对小鼠移植瘤生长及淋巴管生成的影响  

The effects of the D-limonene on the growth of transplantation tumors and lymphangiogenesis in mouse

作　　者：杨慧科[1] 李玉兰[1] 刘慧冬[1] 李晓冬[1] 杨树才[1] 龚春琳[1] 赵玲辉[1] 

机构地区：[1]哈尔滨医科大学基础医学院解剖教研室,哈尔滨150086

出　　处：《中国临床解剖学杂志》2007年第1期57-59,共3页Chinese Journal of Clinical Anatomy

基　　金：黑龙江省科技厅攻关课题(GC03C606-1);黑龙江省卫生厅科学技术课题(2004-170);黑龙江省自然科学基金(200625)

摘　　要：目的:观察右旋柠烯对小鼠移植瘤生长及淋巴管生成的影响,并探讨其作用机制。方法:皮下注射肉瘤(S180)腹水型瘤株构建小鼠移植瘤模型,给予D-Lim onene干预,免疫组化染色观察瘤细胞V EG F-C表达,LY V E-1标记淋巴管,观察其分布。结果:对照组瘤细胞V EG F-C表达较强,瘤周边部淋巴管较多,有淋巴结、肺转移。用药组瘤细胞V EG F-C表达较弱;瘤周边部淋巴管较少,未见淋巴结、肺转移。结论:D-Lim onene有抑制移植瘤内瘤细胞V EG F-C表达和淋巴管生成的作用,有可能降低肿瘤的淋巴道转移。Objective： To explore the effects of D-limonene on the growth and lymphangiogenesis of transplantation tumor in mouse. Methods： The models of mice with transplantation tumor were established by inoculating S 180 cells under skin. The present study, using the immunohistochemical method, investigated the growth of tumor by observing the expression of VEGF-C and the distribution of lymphatic vessels by examining the LYVE-1 mediated by D-Limonene. Results： D-limonene induced a decreased expression of VEGF-C in tumor cells and distribution of lymphatic vessels in periphery of tumor. Furthermore, metastasis was absent in D-limonene group compared with its presence in control group. Conclusions： D-limonene inhibits significantly the expression of VEGF-C, the lymphangiogenesis and metastasis in tumor tissue.

关 键 词：右旋柠烯 移植瘤 淋巴管生成 LYVE-1 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学] R73-36[医药卫生—基础医学]