噻嗪酮对黑斑蛙胚胎发育和蝌蚪生长的影响及血细胞DNA损伤效应  

Effect of Buprofezin on the Growth and Development Inhibition of Tadpole,Rana nigromaculata,and on DNA Damage

作　　者：王坤英[1] 赵俊伟[1] 李宁宁[1] 郭鹏超[1] 袁红旭[1] 周大磊[1] 赵艳红[1] 李卫国[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2007年第4期203-206,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省科技攻关计划(0624410029);河南省教育厅自然科学基金(2006180015);河南省动物学重点学科基金;河南师范大学大学生科研项目

摘　　要：运用体视学方法和单细胞凝胶电泳(single cell gel electrophoresis,SCGE)技术,分析了不同浓度噻嗪酮溶液对黑斑蛙胚胎发育和蝌蚪生长的影响以及对黑斑蛙(Rana nigromaculata)外周血细胞DNA的损伤效应.结果表明,500 mg.L-1和2 000 mg.L-1的扑虱灵(25%噻嗪酮可湿性粉剂)溶液对黑斑蛙蝌蚪的生长发育具有一定程度的抑制作用,并能诱导黑斑蛙外周血细胞DNA出现链断裂损伤.Effects of the insecticide buprofezin, a chitin synthesis inhibitor, on development of embryo and growth of tadpole of frog,Rana nigromaculata, were explored, and the genotoxicity of buprofezin for peripheral haemocyte DNA of Rana nigromaculata with single cell gel electrophoresis （SCGE） were examined, in order to better understand the mechanisms un- derlying DNA damage. The results show that Applaud （25% buprofezin wettable powder） concentration in 500 and 2000mg·L^-1 could lead to the inhibition of growth and development of tadpoles, and induce DNA strand break damage of peripheral haemocyte.

关 键 词：噻嗪酮 黑斑蛙 外周血细胞 单细胞凝胶电泳 细胞毒性 

分 类 号：Q958.116[生物学—动物学] X503.22[环境科学与工程—环境工程]