反相高效液相色谱法测定小儿百部止咳糖浆中橙皮苷含量  

Determination of Hesperidin in Child Baibu Cough Syrup by RP-HPLC

作　　者：赵向阳[1] 

机构地区：[1]安徽省宿州市药品检验所,安徽宿州234000

出　　处：《中国药业》2007年第23期18-19,共2页China Pharmaceuticals

摘　　要：目的建立小儿百部止咳糖浆中橙皮苷的含量测定方法。方法采用反相高效液相色谱法,以乙腈-0.2%磷酸溶液(21∶79)为流动相,检测波长为284nm。结果橙皮苷进样量线性范围为0.35～7.0μg,r=0.9997,平均回收率为98.5%,RSD=0.99%(n=9)。结论该法简便、重现性好,可用于小儿百部止咳糖浆的质量控制。Objective To establish the determination method of hesperidin in Child Baibu Cough Syrup. Methods HPLC was used and methanol-water （71：79） mixture as the mobile phase. The detection wavelength was set at 284 nm. Results Hesperidin showed a good linear relationship at a ranger of 0. 35 - 7.0μg, r = 0. 999 7. The average recovery rate was 98.5%, and RSD was 0. 99% （ n = 9）. Conclusion The method is accurate. It can be used for the quality control of Child Baibu Cough Syrup.

关 键 词：反相高效液相色谱法 小儿百部止咳糖浆 橙皮苷 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]