异核苷低聚核苷酸的合成及其酶促稳定性  

Synthesis and Enzymatic Stability of oligonucleotide Incorporated with Isonucleoside

作　　者：于宏武[1] 张亮仁[1] 马灵台 张礼和[1] 

机构地区：[1]北京医科大学药学院,北京100083

出　　处：《高等学校化学学报》1997年第7期1103-1106,共4页Chemical Journal of Chinese Universities

摘　　要：用磷酸三酯法在溶液中合成了两个带有3’－（S）-胸腺嘧啶基-4’－（R）一羟基-5’－（S）-羟甲基四氢呋喃的三脱氧核苷酸3和4．与三脱氧核苷酸（2）相比，化合物3和4对核酸酶SI具有更大的稳定性．Two trideoxynucleotides incorporated with 3' - (S)- (thymin-1-yl)-4' - (R) -hydroxy-5' -(S)-hydroxymethyl tetrahydrofuran in the 3' end (3) or in center (4) were synthesized via phos-photriester method in solution. Compounds 3 and 4 are stable towards Nuclease S1 compared withthe normal trideoxynucleotides (2).

关 键 词：酶促稳定性 异核 低聚 核苷酸 合成 

分 类 号：O636[理学—高分子化学] O629.11[理学—化学]