联苯胺对大鼠肝脏线粒体同工酶的表达差异研究  

Effect of Benzidine on Expression of Mitochondria Isozyme

作　　者：梅启明[1] 郭鹏[1] 梅秀丽[1] 

机构地区：[1]武汉大学药学院,武汉430072

出　　处：《湖北农业科学》2007年第6期877-879,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金资助项目(20177018)

摘　　要：试验采用不同浓度的联苯胺(50、100、200 mg.kg-1)通过对大鼠进行体内染毒建立毒性作用动物模型。利用聚丙烯酰胺凝胶电泳法分析了大鼠肝脏线粒体SOD、GSH-Px、COX和Ca2+-ATPase同工酶的表达差异。结果表明,同工酶cox-L和ATPase-L只在低浓度联苯胺处理组中特异表达。分析认为,联苯胺可能经体内代谢活化后,对线粒体DNA及其转录和翻译系统产生了作用。Animal model of cancer-inducing effect was build using different concentration of benzidine （50、100、200mg·kg^-1）. The expression differences of isozyme SOD, GSH-Px, COX and Ca^2＋-ATPase in mice liver mitochondria were determined by PAGE. The results showed that isozyme cox-L and ATPase-L could express itself only in the lower isozyme concentration.According to the experiment,the transfer and translation of mitochondria DNA was influenced by activated benzidine.

关 键 词：联苯胺 体内染毒 大鼠肝脏 线粒体 同工酶 表达差异 

分 类 号：O625.13[理学—有机化学]