依那西普对磨屑诱导巨噬细胞分泌肿瘤坏死因子的影响  

Effects of etanercept on tumor necrosis factor secreted by wear debris-induced macrophages

作　　者：陈志荣[1] 张亮[1] 吴兴临[1] 陆志东[1] 金群华[1] 

机构地区：[1]宁夏医学院附属医院骨科,银川750004

出　　处：《国际骨科学杂志》2007年第6期405-406,共2页International Journal of Orthopaedics

基　　金：国家自然科学基金(30560155);教育部科学技术研究重点项目(206161)

摘　　要：目的探讨依那西普(etanercept)对人工关节无菌性松动的影响。方法分离、培养小鼠腹腔巨噬细胞,分为5组。A组为单纯巨噬细胞组,B组为细胞+钛颗粒组,C组为细胞+钛颗粒+10 ng/ml依那西普组,D组为细胞+钛颗粒+100 ng/ml依那西普组,E组为细胞+钛颗粒+1000 ng/ml依那西普组。培养18小时后,用酶联免疫吸附试验检测细胞培养上清液中肿瘤坏死因子含量。结果A、C、D、E组肿瘤坏死因子含量明显低于B组(P<0.001),E组肿瘤坏死因子含量明显低于C组和B组(P <0.001)。结论磨屑可刺激巨噬细胞分泌肿瘤坏死因子,依那西普能够呈剂量依赖地有效抑制磨屑诱导的巨噬细胞分泌肿瘤坏死因子,有望成为预防人工关节无菌性松动的药物。Objective To approach the effect of etanercept on aseptic loosening of prothesis. Methods Mouse peritoneal macrophages were separated and cultivated, there were divided into 5 groups. Group A was treated with macropbages alone, group B with macrophages ＋ Ti debris, group C with macrophages ＋ Ti debris ＋ etanercept （10 ng/ml）, group D with macrophages ＋ Ti debris ＋ etanercept （100 ng/ ml）, group E with macrophages ＋ Ti debris ＋ etanercept （1000 ng/ ml）. After 18h, we are detected the tumor necrosis factor （TNF） in culture supernatants by ELISA. Results The levels of TNF were much lower in group A, C, D, E than those in group B （P〈0. 001 ）, the levels of TNF were much lower in group E than those in group C and B （P〈0. 001 ）. Conclusions The wear debris can stimulate macrophages to secrete TNF,etanercept can significantly inhibited TNF secreted by wear debris-induced macrophages in a dose-dependent manner and hope to be a therapeutic candidate for the prevention of aseptic loosening.

关 键 词：依那西普 巨噬细胞 磨屑 肿瘤坏死因子 

分 类 号：R96[医药卫生—药理学] R687.3[医药卫生—药学]