颉颃细菌GXG-2-2的菌株鉴定及其对植物病原菌的颉颃作用  

Identification of Strain GXG-2-2 and Its Inhibition against Plant Pathogen

作　　者：高晓岗[1] 安德荣[1] 全鑫[1] 马晓东[1] 

机构地区：[1]西北农林科技大学植保学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《西北农业学报》2007年第6期195-198,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：教育部长江学者和创新团队发展计划资助(No.200558)

摘　　要：从枯草中分离筛选获得的颉颃细菌GXG-2-2被鉴定为枯草芽孢杆菌。该菌有很强的分泌胞外蛋白酶的能力。于发酵液中加入60%饱和度的(NH4)2SO4沉淀所得的蛋白粗液对多种植物病原菌有强烈的抑制作用。例如稻瘟病菌(Pyricularia grisea)、小麦赤霉病菌(Fusarium graminearum)、烟草赤星病菌(Alter-naria alternata)、大白菜软腐病菌(Erwinia carotovora subsp.carotovora)等。The antagonistic bacterial strain GXG-2-2 isolated and screened from withered grass was identified as Bacillus subtilis. It has a property of strong producing extracelluar proteinase. A crude antagonistic preparation was obtained by adding ammonium sulfate to the fermented liquid of B. subtilis GXG-2-2 and displayed strong antagonistic activity to various plant pathogenic fungi and bacteria. Such as Fusarium graminearum, Alternaria alternate, Alternaria alternate, Erwinia. carotovora. subsp, carotovora.

关 键 词：枯草芽孢杆菌 鉴定 植物病原菌 颉颃作用 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]