金黄色葡萄球菌L型垂直感染对子代小鼠免疫功能影响的初探  

Immune function on mouse offspring vertical infected by Stpaphylococcus aureus L-forms

作　　者：徐飞[1] 管俊昌[1] 夏佩莹[1] 

机构地区：[1]南京医科大学附属南京儿童医院检验科,南京210008

出　　处：《中国人兽共患病学报》2007年第11期1139-1142,共4页Chinese Journal of Zoonoses

基　　金：安徽省自然科学基金;安徽省教育厅自然科学研究计划项目资助(01043706)(2001KJ172)

摘　　要：目的研究金黄色葡萄球菌L型垂直感染对子代小鼠红细胞免疫、T细胞亚群及NK细胞数量影响。方法采用流式细胞术检测感染后的子代成年小鼠T细胞亚群、NK细胞数量的变化,酵母菌红细胞花环试验法测定红细胞C3b受体花环率(C3bRR)、红细胞免疫复合物花环率(ICR)。结果L型感染组子鼠C3b受体花环率(C3bRR)、红细胞免疫复合物花环率(ICR)降低;子鼠脾脏CD4+细胞明显降低,CD8+细胞明显升高,CD4/CD8的比值降低、NK细胞数量明显降低。结论金葡菌L型垂直感染后对子代免疫功能有一定影响。To explore the effect of Staphylococcus aureus L-forms on erythrocyte immune receptor,T lymphocyte subpopulation and the amount of NK cell in offsprings,T lymphocyte subpopulations of spleen were measured by flow cytometry,and erythrocyte immune functions（including RBC-C3bRR and RBC-ICR）were observed to evaluate the immune function of offspring.The results showeol that the level of splenic CD＋4 T cell in S.aureus L-forms group was lower than that of control group,and the level of CD＋8 T cell was higher than that of control group,so the ratio of CD4/CD8 was inversed ,the level of nature killer cells and the erythrocyte C3bRR,erythrocyte ICR were lower than that of control group.It suggests that S.aureus L-forms can affect the immune function of the mice offsprings to some extent through vertical infection.

关 键 词：金黄色葡萄球菌 细菌L型 免疫功能 

分 类 号：R378.1[医药卫生—病原生物学]