高效液相色谱法快速测定发酵液中的藤黄绿菌素  

The Quick Determination of Pyoluteorin in Fermentaion Broth Using High Performance Liquid Chromatography

作　　者：孙雷[1] 彭华松[1] 程凡[1] 许煜泉[1] 张雪洪[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《上海交通大学学报》2007年第2期325-327,共3页Journal of Shanghai Jiaotong University

基　　金：上海市科技兴农重大攻关项目(2003-3-3-1)

摘　　要：应用反相高效液相色谱,以φ=0.7甲醇为流动相,流速1.0mL/min,紫外检测波长308nm,建立了快速测定荧光假单胞菌M18发酵液中藤黄绿菌素（Pyoluteorin）的方法.实验结果表明,藤黄绿菌素在4.9min出峰,线性范围0.02-1g/L,线性范围良好（r=0.9997）,最低检测浓度0.01g/L,回收率平均为97.7%.The method for the determination of pyoluteorin was founded by employing reversed-phase high performance liquid chromatography. The analysis conditions for the separation of pyoluteorin were achieved as below： 700% methanol as mobile phase, ultraviolet detector （λ=308 nm）, flowing speed 1.0 mL/min. The result shows that the retention time of pyoluteorin is 4.9 min. A good linear relationship exits between the peak area and pyoluteorin concentration ranging from 0.02-1 g/L with correlation coefficient being 0. 999 7. The average recovery is 97.7%. This method provides a base for the production and detection of pyoluteorin.

关 键 词：藤黄绿菌素 高效液相色谱 荧光假单胞菌 

分 类 号：TQ041[化学工程] TQ458