逆转录病毒载体包装细胞的培养及包装过程(英文)  

The culture of retrovirus package cell and the package process

作　　者：赵英会[1] 王继英[2] 张念华[1] 张春山[1] 侯盼飞[1] 

机构地区：[1]泰山医学院,山东泰安271000 [2]山东农科院畜牧所,山东济南250000

出　　处：《泰山医学院学报》2007年第9期734-736,共3页Journal of Taishan Medical College

摘　　要：目的为了获得通用型逆转录病毒载体。方法本实验在成功构建复制缺陷型逆转录病毒载体的基础上,对复制缺陷型逆转录病毒载体的包装细胞进行培养,然后用脂质体介导法将载体转染包装细胞。结果经病毒滴度测定可知包装成功。结论获得了复制缺陷型逆转录病毒载体。Objective： To obtain the replication - defective retrovirus. Methods ： In this research, with the recombined retroviral expression vector was constructed. The package cell was successfully cultured, then the package cell were co - transfected through the recombined vector. Results： The package is finished successfully. Conclusions： the replication - defective retrovirus was produced.

关 键 词：逆转录病毒 包装细胞 包装 

分 类 号：Q78[生物学—分子生物学]