不同去污剂对裙带菜和石莼色素蛋白复合物分离的影响  

Effect of Different Detergents on Separation of Pigment-Protein Complexes from Undaria pinnatifida and Ulva pertusa

作　　者：刘洪艳[1] 

机构地区：[1]天津科技大学海洋科学与工程学院,天津300450

出　　处：《海洋通报》2007年第6期37-41,共5页Marine Science Bulletin

基　　金：国家自然基金项目(30670493);天津市高等学校科技发展基金项目(20040711)资助

摘　　要：以褐藻裙带菜(Undaria pinnatifida)和绿藻石莼(Ulva pertusa)为实验材料,利用SDS,Dig,Triton X-100三种去污剂,分别与叶绿素按不同比例增溶类囊体膜,采用蔗糖密度梯度超速离心的方法分离色素蛋白复合物,蔗糖密度梯度为60%,50%,40%,30%,20%,15%和10%。实验结果表明:当SDS与叶绿素质量比为20∶1时,能够从裙带菜类囊体膜中分离得到5条含蛋白质复合物带。对于石莼类囊体膜的增溶,当SDS与叶绿素的质量比为10∶1,Triton与叶绿素质量为20∶1时,同样能够分离得到5条含蛋白质复合物带。褐藻和绿藻色素蛋白复合物对去污剂的敏感程度有很大差别。The thylakiod membranes isolated from Undaria pinnatifida and Ulva pertusa were solubilized by different detergents SDS, Dig and Triton X-100 at different concentrations. The pigment-protein complexes were separated by centrifugation on the discontinuous sucrose density gradient （ 10 %, 15 %, 20 %, 30 %, 40 %, 50 %, 60 % ）. The results indicated that five pigment-protein complexes were isolated by centrifugation on the discontinuous sucrose density gradient of the SDS-treated Undaria pinnatifida thylakoid membrane. The best separation was obtained with detergent SDS （ SDS ： Chl = 20 ： 1）. Comparatively, SDS （ SDS ： Chl = 10 ： 1 ） and Triton X-100 （ Triton X - 100 ： Chl = 20 ： 1 ） solubilizations equally yielded five pigment-protein complexes. The difference in the sensitivity of brown alga and green alga to the detergent was obvious.

关 键 词：去污剂 色素蛋白复合物 光谱 裙带菜(Undaria pinnatifida) 绿藻石莼(Ulva pertusa) 

分 类 号：Q949.288.5[生物学—植物学]