基于悬浮芯片技术的56种病原微生物的高通量检测  

A high-throughput diagnostic method for detecting pathogenic microbes

作　　者：朱海红[1] 蒋汉梁[1] 陈智[1] 曹清毅[1] 孙箴[1] 侯晓丽[1] 周林福[1] 陈峰[1] 羊正纲[1] 贾红宇[1] 许晓燕[1] 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2007年第6期524-530,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省科技厅重大项目(2003C13015);浙江省医药卫生科学研究基金项目(2006B029)

摘　　要：目的:建立高通量、快速、可靠、经济的病原微生物诊断技术平台。方法:设计和合成针对56种常见病原微生物的探针和阳性对照,采用悬浮芯片技术,对56种病原微生物的阳性标准品进行检测。结果:56种标准品的检测值显著高于阴性对照,各病原微生物的阳性标准品和各探针之间无交叉反应。结论:建立了高通量、快速、可靠、经济的病原微生物诊断技术平台,为突发传染病的病原体诊断提供技术储备。Objective： To develop a high-throughput diagnostic method with suspension array technique for detecting pathogenic microbes. Methods.. The probes and positive controls of 56 kinds of pathogenic microbes were designed,synthesized,and used to detect pathogenic microbes with suspension array technique. Results. Fluorescence signals of 56 positive controls were higher than thos of the negative controls, and there was no cross-reaction between the probes and positive controls of different microbes. Conclusion.. Based on suspension array technique, the high-throughput diagnostic method may be useful in clinical detection of pathogenic microbes.

关 键 词：微生物学技术 基因表达 悬浮芯片技术 高通量诊断 病原微生物 

分 类 号：R446.5[医药卫生—诊断学] R450[医药卫生—临床医学]