酒精性肝病细胞色素P4502E1基因多态性研究  

Study on the association between CYP2E1 enzymes and susceptibility of alcoholic liver disease

作　　者：郭艳梅[1] 王沁[2] 冯爱花[2] 

机构地区：[1]甘肃中医学院临床医疗系,甘肃兰州730000 [2]兰州大学第一医院消化科,甘肃兰州730000

出　　处：《中国实用内科杂志》2007年第24期1949-1951,共3页Chinese Journal of Practical Internal Medicine

基　　金：甘肃省科技攻关计划基金项目(2GS054-A43-014-26)

摘　　要：目的探讨细胞色素P4502E1(CYP2E1)基因多态性与酒精性肝病(ALD)的关系。方法将2004年10月至2005年10月兰州大学第一医院消化科住院及门诊患者80例分为ALD组(40例)和非ALD组(40例)以及健康体检中心103名体检者分为嗜酒组(40名)和正常组(63名)。ALD组与嗜酒组患者均饮酒>80g/d,连续10年以上。非ALD组和正常组均不饮酒。采用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测ALD组、非酒精性肝病组、嗜酒组、正常组中CYP2E1的基因多态性,并进行相关性分析。结果ALD组中C2等位基因型与对照组相比明显升高,P<0.001,差异有显著性意义;在ALD组中随肝纤维化指标异常程度增加,C2基因型明显升高,差异具有显著性意义,P<0.05。结论ALD与CYP2E1基因相关,CYP2E1等位基因C2可能是ALD的危险因素。Objective To investigate the correlation between the CYP2E1 enzymes and alcoholic liver disease. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）method was used to determine polymorphism of CYP2E1. A case control study of 183 subjects was carried out including 40 cases of alcoholic liver disease ,40 alcohol dependent group ,40 nonalcoholic liver disease and 63 controls, Results The frequency of B genotype or C2 allele gene was significantly higher in alcoholic liver disease than in control （ P 〈 0. 001 ） ; the frequency of C2 allele gene was not significantly different between alcoholic liver disease with symptom group and the group without symptom; the frequency of C2 allele gene was increased with the degree of liver fibrosis in alcoholic liver disease group（ P 〈 0. 05 ）. Conclusion C2 gene of CYP2E1 enzymes is related to alcoholic liver disease in Gansu population.

关 键 词：肝疾病 酒精性 细胞色素P4502E1 基因 多态性 聚合酶链反应 

分 类 号：R5[医药卫生—内科学]