螺内酯对大鼠肝纤维化α1(Ⅰ)、α1(Ⅲ)前胶原mRNA表达的影响  

Effects of spironolactone on expression of α1(Ⅰ) and α1(Ⅲ) procollagen mRNA collagen in rat hepatic fibrosis.

作　　者：黄欣[1] 张哲永[1] 曹大春[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院消化科,上海200011

出　　处：《中国实用内科杂志》2007年第24期1960-1961,共2页Chinese Journal of Practical Internal Medicine

摘　　要：目的探讨醛固酮受体拮抗剂螺内酯对大鼠α1(Ⅰ)、α1(Ⅲ)前胶原mRNA表达的影响,评价螺内酯的抗纤维化作用。方法雄性SD大鼠50只,随机分为(1)肝硬化模型组(A、B组各10只):体积分数40%的四氯化碳(CCl4,用精制橄榄油配制),皮下注射,3mL/kg,每周2次,其中A组注射10周,B组注射13周;(2)螺内酯组(C、D组各10只):CCl4注射的同时给予螺内酯20mg/(kg·d)灌胃,其中C组灌胃10周,D组灌胃13周;正常对照组(10只):正常饮食、饮水。分别于第10周末处死A、C组大鼠,13周末处死B、D组及对照组大鼠。逆转录聚合酶链反应(RT-PCR)方法检测大鼠肝脏组织中α1(Ⅰ)、α1(Ⅲ)前胶原mRNA的表达。结果第10周末时,螺内酯组α1(Ⅰ)、α1(Ⅲ)前胶原mRNA表达显著低于肝纤维化模型组(P<0.05)。第13周末时,螺内酯组与肝纤维化模型组相比差异无显著性意义。结论螺内酯对肝纤维化有一定的抑制作用。Objective To investigate the effect of spironolactone on expression of α1（Ⅰ）and α1（Ⅲ） procollagen mRNA in rat hepatic liver fibrosis. Methods Fifty male SD rats were randomly divided into 3 groups. Hepatic fibrosis model group： the rats were injected with 400 mL/L CC14 3 mL/kg subcutaneously two times a week for 10 weeks （ Group A）or 13 weeks（ Group B）. Spironolactone group： the rats were injected with 400 mL/L CC14 3 mL/kg subcutaneously two times a week, Spironolactone equivalent to 20 mg/kg per day was given intragastrically for 10 weeks （ Group C ） or 13 weeks （Group D）, Normal control group： normal chow. The expression of α1（Ⅰ）and α1（Ⅲ） procollagen mRNA were detected by RT-PCR, Results At the end of week 10, the levels of α1（Ⅰ）and α1（Ⅲ） procollagen mRNA were significantly decreased in spironolactone group （P 〈 0. 05 ）, At the end of week 13, there was no significant difference between two groups, Conclusion Spironolactone may have a certain fibrogenesis-inhibiting effect on CCl4-induced hepatic fibrosis.

关 键 词：螺内酯 肝纤维化 前胶原 

分 类 号：R5[医药卫生—内科学]