拟南芥CYCD3;1基因的克隆及功能研究  

Cloning and Functional Studies of CYCD3;1 of Arabidopsis thaliana

作　　者：王慧博[1] 赵剑春[1] 黄守程[1] 李芳[1] 喻富根[1] 

机构地区：[1]南京大学生命科学学院,南京210093

出　　处：《西北植物学报》2007年第11期2153-2157,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30270086)

摘　　要：从拟南芥基因组中克隆出CYCD3;1基因,将其插入植物双元载体pER8中,使其受一个嵌合转录启动子的控制;利用农杆菌介导通过真空渗透法将外源基因导入拟南芥中,经潮霉素抗性筛选出转化植株后,用PCR鉴定出阳性转化植株,对阳性转化植株进行连续光照培养并观察其表型变化,发现转基因株系与野生型之间在抽苔和开花时间上有较大差别。结果表明,CYCD3;1低水平误表达会影响植物的生长发育。CYCD3; 1 is one of the crucial genes controlling the transition of G1 phase to S phase in the cell cycle of eukaryote. In this study,CYCD3;1 was cloned and inserted into pER8 in which it was regulated by a chimeric promoter. Transgenic plantlets were obtained by vacuum infiltration with Agrobacterium infec tion and the positive transformants were screened in 1/2MS supplemented with hygromycin followed by a further PCR identification. The transformants were exposed to 24 h/24 h artificial light and some differ- ences between wild-type plant and transgenic lines in bolting and anthesis were observed. This result showed that low level misexpressing of CYCD3 ; 1 could influence the growth and development of Arabi- dopsis thaliana.

关 键 词：拟南芥 CYCD3 1 基因克隆 生长发育 

分 类 号：Q785[生物学—分子生物学]