大鼠动脉粥样硬化形成过程中凝血酶活化纤溶抑制剂活性与血脂的相关性  

Correlation investigation of TAFI and blood fat in the process of atherosclerosis in the rats

作　　者：陈效安[1] 刘向群[1] 陈焕芹[1] 刘利妍[1] 

机构地区：[1]山东大学齐鲁医院保健科,山东济南250012

出　　处：《中国老年学杂志》2007年第22期2176-2178,共3页Chinese Journal of Gerontology

基　　金：山东省科技发展计划项目(2006GG2202045)

摘　　要：目的探讨在大鼠动脉粥样硬化(AS)形成过程中凝血酶活化纤溶抑制剂(thrombin-activatable fibrinolysis inhibitor,TAFI)活性与血脂的相关性。方法40只健康的雄性Wistar大鼠随机分为4组(每组10只):正常对照组、高脂组、高脂维生素D3(VD3)组、内皮损伤组。分别以普通饲料、高脂饲料、高脂饲料+VD3、高脂饲料+VD3+内皮球囊损伤处理,复制大鼠AS形成过程中的三个阶段的模型,即高脂血症、纤维增生性动脉硬化、较成熟的AS斑块病变。测定血浆总胆固醇(TC)、总甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C),发色底物法测定血浆TAFI的活性。结果与正常对照组比较,三组模型鼠的血浆TC、TG、LDL-C、和TAFI活性明显高于正常对照组(P<0.01),有递增趋势,以内膜损伤组升高最为明显;三组模型鼠的HDL-C水平明显低于正常对照组(P<0.01或P<0.05),有递减趋势,以内膜损伤组降低最为明显;血浆TAFI活性与TC、TG呈正相关。结论AS的发生过程中血脂与血浆TAFI活性呈正相关,TAFI参与了AS的形成过程,其活性升高可能是AS的致病因素之一。Objective To investigate the correlation of thrombin-activatable fibrinolysis inhibitor （TAFI） level in plasma and blood fat in the rats with atherosclerosis （AS）. Methods Totally 40 healthy male Wistar rats were randomly divided into （n = 10）, controls （normal diet）, high lipid, high lipid ＋ vitamin D3, endothelium injure （high lipid ＋ vitamin D overload ＋ sacculus proprius injury） groups, duplicating the three stage models of AS rats, hyperlipoidemia, fiber hyperplasy AS and AS plaque process. Plasma total cholesterol （ TC ）, trig]yeride （TG）, low density lipoprotein-cholesterol （LDL-C） and high density lipoprotein-cholesterol （HDL-C） were detected by Automatic Biochemistry Analyzer. The activity of TAFI was determined by chromogenic substrate assay. Results TC, TG, LDL-C and TAFI activity in plasma of three model groups increased gradually compared with those in the controls （ P 〈 0.01 ）, presenting progressive trend, especially endothelium injure group. HDL-C level in plasma of three model groups decreased gradually compared with that in the controls（P 〈0. 01 or P 〈0. 05）, having decrement trend, especially endothelium injure group. And plasma TAFI activity had positive relation with TG and TC. Conclusions The plasma TAFI activity has positive correlation with blood fat in AS, which indicates that TAFI is one of etiological factors of AS.

关 键 词：动脉粥样硬化 凝血酶活化的纤溶抑制剂 内皮损伤 

分 类 号：R541.4[医药卫生—心血管疾病] R-332[医药卫生—内科学]