应用DNA条码技术鉴定狗咬胶熏料植物种  

STUDY ON PRELIMINARY INSPECTION AND IDENTIFICATION OF THE PATENT PRODUCTS OF DOG CHEWS

作　　者：徐涛[1] 许瑾[1] 李明福[1] 朱水芳[1] 

机构地区：[1]中国检验检疫科学研究院动植物检疫研究所,北京100029

出　　处：《检验检疫科学》2007年第5期13-16,共4页Inspection and Quarantine Science

摘　　要：[目的]利用DNA条码技术对狗咬胶专利产品熏料的植物物种进行了鉴定。[方法]采用CTAB法、CTAB-担体法和磁珠法从狗咬胶表面提取痕量的植物总DNA,对其中植物条码基因rpoB和rpoC1进行分析。[结果]疑似产品的植物熏料不是番薯,可能是旋花科的另一植物。建立了利用植物条码基因进行物种鉴定的实验体系。We identified the plant species of fume materials covered on the dog chews using DNA barcode. The total plant DNA was extracted from the surface of dog chews using the following three methods： CTAB, CTAB-Carrier and magnetics beads by comparison. The analysis results of rpoB and rpoC 1 indicated that the plant specie of fume materials covered on the dog chews was not Ipomoea batatas （L.） Lamarck and was another species （ Convolvulaceae ）. This study established the system on identification of species using plant barcode genes preliminarily, which settled the foundation for the study on inspection technique of biology resource.

关 键 词：条码技术 NA提取 狗咬胶 熏料 物种鉴定 

分 类 号：S41-30[农业科学—植物保护] Q503[生物学—生物化学]