hsBAFF对小鼠脾脏B淋巴细胞增殖与免疫应答及胞内Ca^(2+)水平的影响  

EFFECTS OF SPLENIC B LYMPHOCYTE PROLIFERATION AND RESPONSE AND INTRACELLULAR Ca^(2+) OF hsBAFF IN MICE

作　　者：杨筱曼[1] 单潇潇[1] 陈龙[1] 曹鹏[1] 张双全[1] 

机构地区：[1]南京师范大学生命科学学院江苏省生物医药功能材料工程研究中心,江苏南京210097

出　　处：《中国应用生理学杂志》2007年第4期478-481,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助(30670264);江苏省普通高校自然科学研究计划项目(02KJB180003);江苏省自然科学基金项目(BK2005140)

摘　　要：目的:探讨大肠杆菌表达的人源可溶性B淋巴细胞激活因子hsBAFF对小鼠脾脏B淋巴细胞免疫反应及其对胞内游离Ca2+信号变化的影响。方法:选择20只健康ICR小鼠,雌雄各半,随机分成两组(n=10):①对照组;②hsBAFF实验组。实验组小鼠腹腔注射含hsBAFF(0.1mg/kgbw)的PBS溶液,对照组注射同等剂量的PBS溶液,连续8d。用MTT法检测小鼠脾脏B淋巴细胞的增殖及其对LPS刺激的免疫反应,并用激光共聚焦显微镜分析脾脏B淋巴细胞胞内钙离子水平([Ca2+]i)变化。结果:hsBAFF注射小鼠的脾脏B淋巴细胞增殖和对LPS刺激的免疫反应均明显高于对照组(P<0.05);hsBAFF注射组[Ca2+]i荧光强度维持在相对稳定的高水平上波动,平均荧光强度显著高于对照组(P<0.01),且荧光强度变化率小于对照组。结论:大肠杆菌表达的hsBAFF能促进B淋巴细胞的增殖和免疫应答,从而增强机体免疫功能。hsBAFF激活小鼠脾脏B淋巴细胞可能与[Ca2+]i升高有关。To investigate effects of hsBAFF synthesized in Escherichia coli on spleen B lymphocyte immune response and its intracellular free Ca^2 ＋ （[ Ca^2 ＋ ]i） signaling in mice. Methods： Twenty ICR mice, half males-half females, were chosen and randomly divided into a normal control group（ n= 10） and a hsBAFF treatment group（ n= 10）. The mice in hsBAFF treatment group were given abdominal cavity injection of hsBAFF ,solution which was diluted with phosphate buffered ,saline（PBS） at dosage of 0.1 mg/kg body weight once each day for over eight days. The mice in control group were received abdominal injection of PBS at the same dose and frequency. Spleen B lymphocyte proliferation and its immune response to LPS stimulation in mice were evaluated using an MTT assay, and change of spleen B lymphocyte[Ca^2＋ ]i was assayed under a laser scanning confocal microscope. Results： B lymphocyte pro- liferation and its immune response to LPS stimulation were significantly higher in hsBAFF-treated mice than in control mice （P 〈 0.05）. The B lymphocyte [ Ca^2 ＋ ]i fluorescence intensity in hsBAFF-treated mice maintained at a relatively high level fluctuation, and its average intensity was significantly higher to that of control mice （P 〈 0.01 ）, but change rate of the intensity was lower compared to that of control group. Conclusion： hsBAFF synthesized in Escherichia coli can enhance immune function in the body by increasing B lymphocyte proliferation and its immune response, hsBAFF-activated B lymphocyte function may be associated with in creasing B lymphocytes[ Ca^2＋ ]i.

关 键 词：小鼠 hsBAFF B淋巴细胞 免疫反应 CA^2+ 

分 类 号：R392.11[医药卫生—免疫学]