丹参单体IH764-3通过下调H_2O_2刺激的肝星状细胞FAK水平影响MMP-13和TIMP-1的表达  

EFFECT OF SALVIA MILTIORRHIZA MONOMER IH764-3 ON MMP-13 AND TIMP-1 BY DOWNREGULATING THE EXPRESSION OF FOCAL ADHESION KINASE IN HEPATIC STELLATE CELL STIMULATED BY H_2O_2

作　　者：刘丽[1] 姜慧卿[1] 张晓岚[1] 赵冬强[1] 

机构地区：[1]河北医科大学第二医院消化内科,河北石家庄050000

出　　处：《中国应用生理学杂志》2007年第4期482-486,I0013,共6页Chinese Journal of Applied Physiology

基　　金：河北省科技攻关课题(01276134);河北省自然科学基金资助项目(301361)

摘　　要：目的:研究丹参单体IH764-3对H2O2刺激的肝星状细胞(HSC)基质金属蛋白酶-13(MMP-13)、基质金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响以及此过程中粘着斑激酶(FAK)的变化。方法:应用RT-PCR方法检测MMP-13及FAK mRNA表达,原位杂交方法检测TIMP-1mRNA水平,Western blotting技术检测FAK及TIMP-1蛋白表达。结果:IH764-3干预组的MMP-13mRNA在2h的表达强度明显上调,而TIMP-1mRNA表达明显受抑,FAK mRNA表达强度明显下调;IH764-3干预24h组FAK及TIMP-1蛋白表达受抑制。结论:丹参单体IH764-3可以诱导MMP-13表达,抑制TIMP-1表达,下调FAK表达是其中的机制之一。To investigate the effect of IH764-3 on the expression of MMP-13 and TIMP-1 by H2O2-stimulated hepatic stellate cell and the alteration of FAK during this process. Methods： The expression of MMP-13 and FAIL mRNA was examined by RT-PCR. TIMP-1 mRNA was analyzed by in-situ hybridization. FAK and TIMP-1 were evaluated at protein level through Western blotting method. Results： Being incubated for 2 h, compared with control group, MMP-13 mRNA was upregulated by IH764-3, but TIMP- 1 transcription was reduced in a dose-dependent manner, accompanied with the decrease of FAK mRNA. The expression of TIMP-1 and FAK protein in HSC also decreased after being exposed by IH764-3 for 24 h. Conclusion： IH764-3 can induce the expression of MMP-13 and inhibit the expression of TIMP-1. Down-regulating the expression of FAK mRNA may be one of its mechanisms.

关 键 词：丹参单体IH764—3 肝星状细胞 基质金属蛋白酶-13 粘着斑激酶 基质金属蛋白酶组织抑制因子-1 

分 类 号：R541.3[医药卫生—心血管疾病]