有氧运动中NADPH氧化酶介导产生的活性氧对大鼠神经胶质细胞活力的影响  

Effects of ROS Mediated by NADPH Oxidase on Cell Survival in Rat Astrocytes after Aerobic Exercise

作　　者：董静梅[1] 郭层城[2] 

机构地区：[1]兰州城市学院体育系,甘肃兰州730070 [2]西北师范大学体育学院,甘肃兰州730070

出　　处：《中国体育科技》2007年第6期118-121,共4页China Sport Science and Technology

摘　　要：建立大鼠有氧运动模型,原代培养了有氧运动后大鼠神经胶质细胞,阐明有氧运动后类NADPH氧化酶介导产生的活性氧(ROS)对细胞正常生理功能的影响。通过实验发现,有氧运动后大鼠神经胶质细胞中O2.-和H2O2生成均明显增加,而人工饲喂NADPH氧化酶的抑制剂二联苯碘(diphenylene iodonium,DPI)或夹竹桃麻素(apocynin,APO)后,均明显地降低由运动引起的大鼠神经胶质细胞O2.-和H2O2生成量;细胞活性的测定显示,DPI、APO、超氧化物歧化酶+过氧化氢酶均能明显降低运动后大鼠神经胶质细胞活性。表明有氧运动中类NADPH氧化酶介导产生的ROS是大鼠神经胶质细胞生存所必需,过度进补抗氧化剂将干扰细胞的正常生理活动甚至导致细胞死亡。Setting up the model of rat aerobic exercise, using primary culture of rat astrocyte as the materials,the purpose of this study was to determine whether phagocyte-like NADPH oxidase plays a role in ROS generation and cell survival in rat astrocytes after aerobic exercise. We found that the release of ROS in rat astrocytes was significantly increased after aerobic exercise. Further study indicated that low dose breeding with diphenylene iodonium （DPI） or apocynin （APO）, two inhibitors of NADPH oxidase,significantly suppressed ROS release caused by aerobic exercise. Moreover,Cell survival assay showed that DPI,APO, catalase plus superoxide dismutase concentration and time-dependently decreased the survival of normal astrocytes. The result suggested that in the aerobic exercise, ROS generation mediated by phagocyte-like NADPH oxidase play a critical role in astrocytes survival. Antioxidants supplement excessively will interfere with the normal cell functions even result the cell death in the aerobic exercise.

关 键 词：有氧运动 NADPH氧化酶 活性氧 星形神经胶质细胞 细胞活性 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]