雷公藤内酯醇在血管内皮细胞增殖移行中对u-PA、PAI-1蛋白表达的影响  

Effect of triptolide on protein expression of u-PA、PAI-1 of vascular endothelial cells in proliferation and migration

作　　者：王清秀[1] 王佃科[1] 张杰[1] 曹永亮[1] 王杰[1] 

机构地区：[1]潍坊医学院附属医院眼科中心,潍坊261031

出　　处：《中国中医眼科杂志》2007年第6期339-341,共3页China Journal of Chinese Ophthalmology

基　　金：山东省教育厅重点资助课题(No.J01K08)

摘　　要：目的研究雷公藤内酯醇对人脐静脉血管内皮细胞体外培养增殖过程中纤溶酶原激活物系统蛋白表达的抑制作用。方法体外培养传三代人脐静脉内皮细胞(human umbilical vein endothelial cells,HU-VECs),在细胞增殖的同时分别加入不同浓度的雷公藤内酯醇,以免疫组织化学方法检测内皮细胞u-PA、PAI-1蛋白表达量。结果免疫组织化学染色表明雷公藤内酯醇明显抑制内皮细胞分泌U-PA、PAI-1的作用,治疗组均明显低于对照组(P<0.01);并呈剂量依赖性,随其浓度增加抑制作用加强。结论雷公藤内酯醇可明显抑制血管内皮细胞u-PA、PAI-1蛋白表达,从而有效抑制血管内皮细胞的增殖移行。OBJECTIVE To investigate the inhibitory effects of triptolide on the protein expression of members of the plasminogen/plasminogen activator system in endothelial cells cultured in vitro. METHODS Human umbilical vein endothelial cells（HUVECs）were cultured in vitro and the passage 3 cells treated with triptolide.The effects of triptolide on endothelial u - PA and PAI- 1 secretion were investigated by the technique of immunohistochemical stain. RESULTS Triptolide suppressed the protein expression of u - PA and PAI- 1 in dose - dependent manner;Them were significant lesser than the control group（ P 〈 0.01 ）. CONCLUSIONS Triptolide could resist the protein expression of u - PA, PAI- 1 of vascular endothelial cells in proliferation and migration, so it can inhibit the proliferation and migration of vascular endothelial cells effectively.

关 键 词：雷公藤内酯醇 血管内皮细胞 蛋白表达 血管生成 

分 类 号：R285.5[医药卫生—中药学]