脂溢性角化病皮损区受体活化Smad的变化  

Down-regulation of receptor-activited Smads in seborrheic keratosis

作　　者：杨扬[1] 何威[1] 李颖[1] 何云志[1] 黄海[1] 林自华[1] 吴军[1] 杨进清[1] 

机构地区：[1]第三军医大学新桥医院皮肤科,重庆400037

出　　处：《第三军医大学学报》2007年第23期2219-2222,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30371298)~~

摘　　要：目的探讨转化生长因子β(transforming growth factor-β,TGF-β)信号转导通路中受体活化Smads(Smad2,Smad3)在脂溢性角化病皮损区的表达及其意义。方法采用反转录-实时定量聚合酶链式反应(RT and quantitative Real-Time PCR)和免疫组化技术分别检测脂溢性角化病皮损和正常对照皮肤中Smad2及Smad3的mRNA和Smad1/2/3及磷酸化Smad2/3(p-Smad2/3)蛋白的表达情况。结果与正常对照皮肤组相比,脂溢性角化病皮损中Smad2、Smad3的mR-NA以及Smad1/2/3、p-Smad2/3的表达水平下降。结论脂溢性角化病中受体活化Smads(Smad2、Smad3)和p-Smad2/3表达下调可阻断TGF-β信号转导,可能使TGF-β抑制上皮增生的作用不能有效发挥,从而促进了脂溢性角化病表皮的过度增生。Objective To investigate the expression of receptor-activited Smads （Smad2, Smad3 ） and phosphorylated Smad2,3 （p-Smad 2/3） in seborrheic keratosis. Methods The skin tissues of seborrheic ker- atosis from 45 patients （25 males and 20 females, average age： 57.6 years, average course of disease： 3.8 years） who received no treatment before and normal skin tissues from 40 patients （22 males and 18 females, average age ： 48 years） undergoing plastic surgery in our hospital were examined for the mRNA levels of Smad2 and Smad3 by quantitative real-time PCR, and the protein levels of Smadl/2/3 and p-Smad2/3 by immunohistochemistry. Results The mRNA levels of receptor-activited Smads （ Smad2, Smad3 ） and the protein levels of Smadl/2/3, p-Smad2/3 were significantly down-regulated in the skin tissues of seborrheic keratosis in com- parison with control tissues. Conclusion Down-regulation of receptor-activited Smads （Smad2, Smad3 ） and p-Smad2/3 might interfere TGF-β signal transduction pathway and contribute to formation of aberrant hyperplastic epidermis in seborrheic keratosis.

关 键 词：脂溢性角化病 转化生长因子Β 受体活化Smad SMAD2 SMAD3 p—Smad2/3 信号通路 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学] R730.23[医药卫生—基础医学]