neuB1失活空肠弯曲菌O∶19突变株感染致病力的初步研究  

Pathogenicity of a mutant from Campylobacter jejuni O∶19 by deleting neuB1

作　　者：向淑利[1] 蔡方成[2] 张晓萍[2] 

机构地区：[1]泸州医学院附属医院神经内科,泸州646000 [2]重庆医科大学附属儿童医院儿科研究所,重庆400014

出　　处：《第三军医大学学报》2007年第23期2223-2225,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30170990)~~

摘　　要：目的探讨neuB1失活空肠弯曲菌(Campylobacter jejuni,CJ)O∶19突变株的感染致病力。方法由CJ O∶19野生株构建neuB1灭活的突变株,检测其对正常人血清杀菌活性的敏感性、运动力和自身凝集(AAG)活性,并与野生株进行比较。结果突变株对10%正常成人血清(normal human serum,NHS)杀菌活性的敏感性高于同源的野生株(P<0.05),孵育15min和60min后突变株存活率分别为(20.6±7.4)%和(9.6±3.6)%,而野生株分别为(36.9±5.9)%和(15.5±4.3)%,但突变株的运动力和AAG活性与野生株比较却没有显著性差异(P>0.05)。结论neuB1失活CJ O∶19突变株仍具有较强的感染致病力,因而应考虑灭活后突变菌株疫苗的开发研究。Objective To explore the pathogenicity of Campylobacter jejuni （C J） O： 19 strain after deletion of neuB1. Methods The mutant of CJ O： 19 by deleting neuB1, which LPS is deficient in sialic acid, was compared with the homologous wild strain by assaying their sensitivity to bactericidal activity, motility and autoagglutination of 10% normal human serum （NHS）. Results The sensitivity of the mutant strain to bactericidal activity of serum was superior to that of the wild strain. The survival rate of the mutant strain was （20.6 ± 7.4）% and （9.6 ±3.6）% after incubation with 10% NHS for 15 min and 60 min, that of wild strain was （36.9 ±5.9）% and （15.5 ± 4.3 ）% respectively. The wild and mutant strains showed no significant difference in sensitivity to motility and autoagglutination of NHS. Conclusion The mutant strain possesses remarkable pathogenicity so that an inactivated

关 键 词：空肠弯曲菌 感染致病力 运动力 自身凝集活性 突变株 

分 类 号：R378[医药卫生—病原生物学] R392[医药卫生—基础医学]