粘帚霉对核盘菌菌核的寄生作用及其细胞壁降解酶活性分析  

Studies on the Cell Wall Degrading Enzymes during the Mycoparasitism of Gliocladium spp.Isolates with the Sclerotia of Sclerotinia sclerotiorum

作　　者：马桂珍[1] 高会兰[2] 张拥华[2] 李世东[2] 谢丙炎[3] 

机构地区：[1]淮海工学院海洋学院,连云港222005 [2]中国农业科学院植物保护研究所,北京100081 [3]中国农业科学院蔬菜研究所,北京100081

出　　处：《吉林农业大学学报》2007年第6期628-632,共5页Journal of Jilin Agricultural University

基　　金：国家"863"计划项目(2003AA246010)

摘　　要：在培养皿中测定粘帚霉对核盘菌菌核的寄生作用,结果表明:不同粘帚霉菌株对核盘菌菌核具有较强的寄生作用,寄生率均达到85%以上。HL-1-1菌株的寄生率最高,为100%,其次为菌株SH-1-1、SYP-1-3和SS-1-1。供试粘帚霉菌株使核盘菌菌核软化,最后导致腐烂。平板透明圈法和比色法测定粘帚霉的细胞壁降解酶活性结果表明:供试的粘帚霉菌株菌能产生蛋白酶、几丁质酶、葡聚糖酶和纤维素酶,其寄生菌核能力越强,蛋白酶、几丁质酶和葡聚糖酶的酶活性越高。表明在粘帚霉侵染核盘菌菌核的过程中,蛋白酶、几丁质酶和葡聚糖酶起着重要作用。Mycoparasitism of some isolates of Gliocladium spp： with the sclerotia of Sclerotinia sclerotiorum were studied. Results indicated that the isolates showed strong parasite ability with the sclerotia and the average of parasite rate were above 85 %. Among them, the parasite rate of HL - 1 - 1 strain was 100% and those of SH - 1 - 1, SYP - 1 - 3 and SS - 1 - 1 followed. Mycoparasitism with the Gliocladium spp. isolates tested led the sclerotia issue soft and rot at the end. Some cell wall degrading enzymes including the proteinase, chitinase, glucanase and cellulase were induced. The higher the parasite ability is, the stronger the activity of the former three enzymes are. It showed that the proteinase, chitinase and glucanase played an important role during the mycoparasitism process.

关 键 词：粘帚霉 核盘菌 菌核 寄生作用 细胞壁降解酶 

分 类 号：S432.44[农业科学—植物病理学]